Mass production of insect pathogenic hypocreale fungi, Metarhizium anisopliae on solid substrates and liquid media

Q4 Agricultural and Biological Sciences Journal of Biological Control Pub Date : 2023-06-14 DOI:10.18311/jbc/2022/30970
E. Sowmya, A. Hosamani, M. Mamatha
{"title":"Mass production of insect pathogenic hypocreale fungi, Metarhizium anisopliae on solid substrates and liquid media","authors":"E. Sowmya, A. Hosamani, M. Mamatha","doi":"10.18311/jbc/2022/30970","DOIUrl":null,"url":null,"abstract":"Mass production of Metarhizium anisopliae on different locally available solid substrates viz., pearl millet, maize, sweet corn, cowpea, rice bran, wheat bran, press mud + yeast extract (1%) and bagasse + yeast extract (1%) and Pulses viz., green gram, red gram, chick pea and cowpea and liquid media viz., sabouraud’s dextrose broth, potato dextrose broth, sabouraud’s maltose broth, sabouraud’s sucrose broth and sabouraud’s glucose broth was taken up. Significantly highest number of CFU were recorded in green gram, 94.00, 91.00 and 97.00 CFU per ml with 13.24 × 108 , 16.42 × 108 and 15.56 × 108 conidia per ml in 3 promising strains, UASRBC-Ma2, UASRBC-Ma31 and ICAR-NBAIRMa4, respectively followed by wheat bran, 78.00, 73.00 and 93.33 CFU per ml with 22.00 × 108 , 26.23 × 108 and 24 × 108 conidia per ml and maximum dry mycelia recorded in Sabouraud’s maltose yeast broth, 127.33 mg, 219.67 mg, 325.67 mg and 386.00 mg per 100 ml of liquid broth on 4th, 6th, 8th and 10th days after inoculation. The present study revealed that solid substrates namely green gram, rice and wheat bran and liquid media, sabouraud’s maltose broth provide a simplest productive medium for mass production of Metarhizium anisopliae spores.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"68 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biological Control","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18311/jbc/2022/30970","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

Abstract

Mass production of Metarhizium anisopliae on different locally available solid substrates viz., pearl millet, maize, sweet corn, cowpea, rice bran, wheat bran, press mud + yeast extract (1%) and bagasse + yeast extract (1%) and Pulses viz., green gram, red gram, chick pea and cowpea and liquid media viz., sabouraud’s dextrose broth, potato dextrose broth, sabouraud’s maltose broth, sabouraud’s sucrose broth and sabouraud’s glucose broth was taken up. Significantly highest number of CFU were recorded in green gram, 94.00, 91.00 and 97.00 CFU per ml with 13.24 × 108 , 16.42 × 108 and 15.56 × 108 conidia per ml in 3 promising strains, UASRBC-Ma2, UASRBC-Ma31 and ICAR-NBAIRMa4, respectively followed by wheat bran, 78.00, 73.00 and 93.33 CFU per ml with 22.00 × 108 , 26.23 × 108 and 24 × 108 conidia per ml and maximum dry mycelia recorded in Sabouraud’s maltose yeast broth, 127.33 mg, 219.67 mg, 325.67 mg and 386.00 mg per 100 ml of liquid broth on 4th, 6th, 8th and 10th days after inoculation. The present study revealed that solid substrates namely green gram, rice and wheat bran and liquid media, sabouraud’s maltose broth provide a simplest productive medium for mass production of Metarhizium anisopliae spores.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
在固体基质和液体培养基上大量生产昆虫致病性绿僵菌
在当地可获得的不同固体基质(珍珠粟、玉米、甜玉米、豇豆、米糠、麦麸、榨汁泥+酵母提取物(1%)和甘蔗渣+酵母提取物(1%)、豆类(绿克、红克、鹰嘴豆和豇豆)和液体培养基(沙伯劳德葡萄糖肉汤、马铃薯葡萄糖肉汤、沙伯劳德麦芽糖肉汤、沙伯劳德蔗糖肉汤和沙伯劳德葡萄糖肉汤)上大量生产金龟子绿僵菌。明显最多的CFU记录在绿豆,94.00,91.00和97.00与13.24×108 CFU /毫升,16.42×108和15.56×108每毫升3有前途的菌株分生孢子,UASRBC-Ma2, UASRBC-Ma31 ICAR-NBAIRMa4,分别其次是麦麸,78.00,73.00和93.33与22.00×108 CFU /毫升,每毫升26.23×108和24×108分生孢子和最大干菌丝记录在Sabouraud麦芽糖发酵肉汤,127.33毫克,219.67毫克,接种后第4、第6、第8、第10天,每100 ml液体肉汤中分别含有325.67 mg和386.00 mg。本研究表明,绿克、稻谷、麦麸等固体基质和麦芽糖汤等液体培养基为绿僵菌孢子的大规模生产提供了最简单的生产培养基。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of Biological Control
Journal of Biological Control Agricultural and Biological Sciences-Insect Science
CiteScore
0.50
自引率
0.00%
发文量
10
期刊最新文献
Novel records of parasitoids targeting fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), in rice-corn-based cropping systems in the Philippines Diversity of phytophagous and predatory mites in vegetable crops in Himachal Pradesh, India Apivectoring: Harnessing pollinators for sustainable crop protection and pollination In vitro evaluation of microencapsulated Bacillus thuringiensis (Berliner) formulation against Helicoverpa armigera (Hubner) Evaluating the efficiency of silver nanoparticles prepared using Pseudomonas fluorescens and Bacillus thuringiensis subsp. tenebrionis in controlling eggs and adults of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1