Human placental alkaline phosphatase covalently immobilized on a cross-flow microfiltration polyvinylidene difluoride membrane. Part I. Physicochemical effectors

Q4 Chemical Engineering 分子催化 Pub Date : 1994-09-27 DOI:10.1016/0304-5102(94)00078-6

M.G. Roig , J.F. Bello , S. Rodriguez , J.M. Cachaza , J.F. Kennedy

引用次数: 2

Abstract

Human placental alkaline phosphatase has been chemically immobilized on a hydrophilic cross-flow microfiltration membrane made of polyvinylidene difluoride (PVDF) chemically labelled with 1-carbonylimidazole groups. Physicochemical characterization of this immobilized biocatalyst focused especially on attributes such as the immobilization isotherms (Langmuir type) of the enzyme to the support, the stability of the catalytic activity, the effects of pH and temperature on this activity and the existence of limitations of external diffusion for H⁺, substrate and/or products. Regarding enzyme stability and its dependence on different experimental conditions, patterns of hysteresis or memory are proposed.

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人胎盘碱性磷酸酶在交叉流微滤聚偏二氟乙烯膜上的共价固定化。第一部分:物理化学效应物

将人胎盘碱性磷酸酶化学固定在以1-羰基咪唑为化学标记的聚偏二氟乙烯(PVDF)制成的亲水性交叉流微滤膜上。该固定化生物催化剂的物理化学表征主要集中在酶对载体的固定化等温线(Langmuir型)、催化活性的稳定性、pH和温度对该活性的影响以及H+、底物和/或产物的外部扩散是否存在限制等属性上。针对酶的稳定性及其对不同实验条件的依赖性，提出了滞回模式或记忆模式。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

分子催化 Chemical Engineering-Catalysis

CiteScore

1.50

自引率

0.00%

发文量

2959

期刊介绍： Journal of Molecular Catalysis (China) is a bimonthly journal, founded in 1987. It is a bimonthly journal, founded in 1987, sponsored by Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, under the supervision of Chinese Academy of Sciences, and published by Science Publishing House, which is a scholarly journal openly circulated both at home and abroad. The journal mainly reports the latest progress and research results on molecular catalysis. It contains academic papers, research briefs, research reports and progress reviews. The content focuses on coordination catalysis, enzyme catalysis, light-ribbed catalysis, stereochemistry in catalysis, catalytic reaction mechanism and kinetics, the study of catalyst surface states and the application of quantum chemistry in catalysis. We also provide contributions on the activation, deactivation and regeneration of homogeneous catalysts, solidified homogeneous catalysts and solidified enzyme catalysts in industrial catalytic processes, as well as on the optimisation and characterisation of catalysts for new catalytic processes. The main target readers are scientists and postgraduates working in catalysis in research institutes, industrial and mining enterprises, as well as teachers and students of chemistry and chemical engineering departments in colleges and universities. Contributions from related professionals are welcome.

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