The protective effects of apigenin on OLN-93 cellular model of cerebral stroke

IF 0.3 Q4 PHYSIOLOGY Physiology and Pharmacology Pub Date : 2021-09-01 DOI:10.52547/ppj.25.3.270

Samira Miraee, M. Farhadi, S. B. Jameie, Shabnam Najafie

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引用次数: 1

Abstract

Introduction: Cerebral stroke is known to be the third most common cause of death in the world. To study pathophysiology and effects of the therapeutic agents on stroke, the cellular model of stroke recently was used more. ONL-93, oligodendrocyte like cell, is known as an appropriate model to study the role of glial cells in stroke. Apigenin is a flavonoid that has neuroprotective and neurogenic effects; therefore, the purpose of this study was to investigate the role of apigenin flavonoid on the OLN-93 cell line in terms of oxygen and glucose deprivation in the cellular stroke model. Methods: The cells were divided into experimental, negative and positive control groups. Then, MTT, reactive oxygen species (ROS), annexin and propidium iodide as well as Western blotting assays were performed to evaluate the viability and apoptosis. Results: The results showed that there was a significant increase in the number of live cells following administration of 1μM of apigenin in experimental groups and also, there was a significant difference in the number of live cells between two doses of 1μM and 0.75μM of the apigenin. The amount of ROS produced at a concentration of 1μM apigenin was a significant decrease compared to the positive control group and apoptotic cells also decreased significantly. The results for the expression of P53 protein showed a significant reduction in experimental groups. Conclusion: Based on our results, apigenin could have beneficial effects through the reduction of P53 and ROS production.

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芹菜素对脑卒中OLN-93细胞模型的保护作用

导读:脑中风是世界上第三大常见死亡原因。为了研究脑卒中的病理生理及药物对脑卒中的影响，近年来脑卒中细胞模型得到了广泛的应用。ONL-93，少突胶质细胞样细胞，被认为是研究神经胶质细胞在中风中的作用的合适模型。芹菜素是一种黄酮类化合物，具有神经保护和神经原性作用;因此，本研究的目的是探讨芹菜黄酮在细胞性脑卒中模型中对OLN-93细胞系缺氧和葡萄糖剥夺的作用。方法:将细胞分为实验组、阴性对照组和阳性对照组。然后采用MTT、活性氧(ROS)、膜联蛋白(annexin)和碘化丙啶(propidium iodiide)以及Western blotting检测细胞活力和凋亡情况。结果:给药1μM的芹菜素后，实验组的活细胞数量明显增加，且1μM和0.75μM的芹菜素两种剂量的活细胞数量有显著差异。芹菜素浓度为1μM时，ROS生成量较阳性对照组明显减少，凋亡细胞数量也明显减少。结果显示，实验组P53蛋白表达明显降低。结论:根据我们的研究结果，芹菜素可能通过减少P53和ROS的产生而具有有益的作用。

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来源期刊

Physiology and Pharmacology PHYSIOLOGY-

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0.70

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0.00%

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期刊介绍： Physiology and Pharmacology is the official English publication of the Iranian Society of Physiology and Pharmacology. The journal publishes Review articles, Full-length original articles, Letter to editor and Short communications in physiology, pharmacology and related subjects. The aim of this journal is to provide a medium of scientific communication for investigators in the field of Physiology and Pharmacology. The editors will welcome original basic and applied research articles from Physiologists and Pharmacologists. Articles should be in English language. The papers submitted to this journal must not be Published or under consideration for publication elsewhere. Physiology and Pharmacology is an open access journal which means that all contents is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search or link to the full text of the articles in this journal without asking prior permission from the publisher or the author.