Sex Difference in Expression of Metallothionein mRNA in Mouse Liver after Low Dose Cadmium Injection

T. Fujioka, N. Sogawa, C. Sogawa, N. Oda, H. Furuta
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引用次数: 1

Abstract

Environmental pollution caused by cadmium (Cd) has been an object of public concern for considerable time. Among heavy metals, both Cd and mercury are well known to be severe toxic to living tissue. Interestingly, it has been suggested that there are sex differences in Cd effects1-4). For example, blood pressure in male rats was elevated by administration of Cd, but not in females2). Itai-Itai disease, that has been considered a chronic symptom of Cd poisoning, was reported in old women, especially5). However, there is no consensus whether Cd effects are more severe in males or females, so a sex difference in the effect of Cd has remained obscure. Since Margoshes, et al.6) reported that low molecular weight proteins from horse kidney cortex contained abundant Cd and Kagi, et al.7) identified these proteins as metallothionein (MT), there have been many discussions concerning the functions of MT. According to reports suggesting that MT is induced in animal tissues by Cd administration and noting a positive correlation between dose-dependent increases in hepatic MT and Cd LD50, the detoxification of heavy metal has been considered one of the main functions of MT8). Therefore, an investigation of differences in MT synthesis by Cd in male and female animals should be of considerable interest for evaluating sex differences in the influences of Cd. In this study, we investigated MT mRNA expressions in the livers of male and female mice after Cd injections using the RT-PCR method.
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低剂量镉注射后小鼠肝脏金属硫蛋白mRNA表达的性别差异
长期以来,镉对环境的污染一直是公众关注的问题。众所周知,在重金属中,镉和汞对活组织都有严重的毒性。有趣的是,有人认为Cd效应存在性别差异(1-4)。例如,服用Cd后,雄性大鼠的血压升高,而雌性大鼠则没有。Itai-Itai病被认为是Cd中毒的一种慢性症状,在老年妇女中有报道(5)。然而,关于Cd对男性还是女性的影响更严重,目前还没有共识,因此Cd影响的性别差异仍然不清楚。自从Margoshes等人(6)报道马肾皮质的低分子量蛋白含有丰富的Cd, Kagi等人(7)将这些蛋白鉴定为金属硫蛋白(MT)以来,人们对MT的功能进行了许多讨论。据报道,MT在动物组织中由Cd诱导,并注意到肝脏MT和Cd LD50的剂量依赖性增加之间存在正相关关系。重金属的解毒一直被认为是MT8的主要功能之一。因此,研究雄性和雌性动物中Cd合成MT的差异对于评估Cd影响的性别差异具有重要意义。在本研究中,我们使用RT-PCR方法研究了注射Cd后雄性和雌性小鼠肝脏中MT mRNA的表达。
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