Forked end: a novel transmembrane protein involved in neuromuscular specificity in drosophila identified by gain-of-function screening.

T. Umemiya, Etsuko Takasu, M. Takeichi, T. Aigaki, A. Nose
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引用次数: 12

Abstract

The Drosophila neuromuscular connectivity provides an excellent model system for studies on target recognition and selective synapse formation. To identify molecules involved in neuromuscular recognition, we conducted gain-of-function screening for genes whose forced expression in all muscles alters the target specificity. We report here the identification of a novel transmembrane protein, Forked end (FEND), encoded by the fend gene, by the said screening. When the FEND expression was induced in all muscles, motoneurons that normally innervate muscle 12 formed ectopic synapses on a neighboring muscle 13. The target specificity of these motoneurons was also altered in the loss-of-function mutant of fend. During embryonic development, fend mRNA was detected in a subset of cells in the central nervous system and in the periphery. These results suggest that FEND is a novel axon guidance molecule involved in neuromuscular specificity.
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分叉端:一种新的跨膜蛋白参与神经肌肉特异性在果蝇的功能获得筛选鉴定。
果蝇的神经肌肉连通性为目标识别和选择性突触形成的研究提供了一个很好的模型系统。为了鉴定参与神经肌肉识别的分子,我们对在所有肌肉中强制表达改变靶特异性的基因进行了功能获得筛选。通过上述筛选,我们报道了一种新的跨膜蛋白,分叉端(fende),由fende基因编码。当在所有肌肉中诱导表达时,通常支配肌肉12的运动神经元在邻近肌肉13上形成异位突触。这些运动神经元的靶特异性在功能丧失突变体中也发生了改变。在胚胎发育过程中,在中枢神经系统和外周细胞的一个亚群中检测到fendmrna。这些结果表明,残端残端残端残端残端残端残端残端残端残端残端是参与神经肌肉特异性的新型轴突引导分子。
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