{"title":"Expression of Amino-terminal Fragment of Clostridium chauvoei toxin A","authors":"K. Karthik, M. Mashooq, V. Nagaleekar","doi":"10.5958/0973-9149.2019.00007.8","DOIUrl":null,"url":null,"abstract":"Clostridium chauvoei, the causative of black quarter in ruminants, is an anaerobic spore forming organism, produces different types of exotoxins. Clostridium chauvoei toxin A (cctA) is an important toxin possessing hemolytic and cytotoxic property and also claimed to be a protective antigen. Characterization of the different regions of the toxin has not been carried out yet. Hence, the regions responsible for different activities of the toxin are not clear. As an initial step, amino-terminal region (34-75aa) of cctA gene was cloned and expressed in pRham-N-His-Sumo-Kan vector. Expressed protein was confirmed by SDS-PAGE analysis.","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"19 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Immunology and Immunopathology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5958/0973-9149.2019.00007.8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Clostridium chauvoei, the causative of black quarter in ruminants, is an anaerobic spore forming organism, produces different types of exotoxins. Clostridium chauvoei toxin A (cctA) is an important toxin possessing hemolytic and cytotoxic property and also claimed to be a protective antigen. Characterization of the different regions of the toxin has not been carried out yet. Hence, the regions responsible for different activities of the toxin are not clear. As an initial step, amino-terminal region (34-75aa) of cctA gene was cloned and expressed in pRham-N-His-Sumo-Kan vector. Expressed protein was confirmed by SDS-PAGE analysis.
chauvoei梭状芽胞杆菌是一种厌氧孢子形成的生物，是反刍动物黑斑病的病原体，产生不同类型的外毒素。chauvoei Clostridium toxin A (cctA)是一种重要的毒素，具有溶血和细胞毒性，被认为是一种保护性抗原。目前还没有对毒素的不同区域进行表征。因此，负责毒素不同活动的区域并不清楚。首先克隆cctA基因氨基末端区(34-75aa)，并在pRham-N-His-Sumo-Kan载体中表达。通过SDS-PAGE分析证实表达蛋白。
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