EVALUATING THE VOLATILE COMPOUNDS DURING ALCOHOLIC FERMENTATION OF DIFFERENT SACCHAROMYCES CEREVISIAE STRAINS IN MIXED JUICES OF CASHEW APPLE AND LONGAN

Kanokchan Sanoppa
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Abstract

The purpose of this research was to evaluate the microbiological, chemical, and volatile compounds of mixed juices comprised of cashew apple and longan fermented with two yeast strains of Saccharomyces cerevisiae (C12 and EC1118). The two fermentations in the mixed juices were similar physicochemical parameters (the growth of yeast, pH, total acidity, total soluble solids, and total sugar). The most abundant of the volatile compounds in these wines were ethyl acetate, ethyl hexanoate, ethyl octanoate, ethyl decanoate, isoamyl acetate, ethanol, isobutanol, isoamyl alcohol, 2-phenylethanol, β-ocimene, and acetic acid. Seven volatile compounds in the wines had odor activity values (OAVs) higher than 1, and also were selected and quantified (ethyl octanoate, ethyl hexanoate, ethyl decanoate, isoamyl acetate, isoamyl alcohol, 2-phenylethanol, and linalool). Ethyl octanoate (fruity, floral, and pineapple) had the highest OAVs in both of the mixed juices fermented with the yeast strains C12 and EC1118. The S. cerevisiae strain EC1118 can significantly produce the highest total volatile contents (359.23 mg/L), and we also found a higher level of total ester (95.50 mg/L) in the wines. Additionally, the S. cerevisiae strain EC1118 can provide a higher content of fruity aroma in wines, as indicated by the calculation of OAVs. The sensory analysis of the wines was acceptable to all the panelists, who gave color, clarity, taste, flavor, and overall acceptance scores as “like slightly” to “like moderately.”
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不同酿酒酵母菌对腰果、苹果、桂圆混合汁酒精发酵挥发性物质的影响
研究了用两株酿酒酵母(C12和EC1118)发酵腰果苹果和龙眼混合果汁的微生物学、化学和挥发性成分。混合果汁中两种发酵的理化参数(酵母生长、pH、总酸度、总可溶性固形物和总糖)相似。这些酒中最丰富的挥发性化合物是乙酸乙酯、己酸乙酯、辛酸乙酯、癸酸乙酯、乙酸异戊酯、乙醇、异丁醇、异戊醇、2-苯乙醇、β-辛烯和乙酸。选取了辛酸乙酯、己酸乙酯、癸酸乙酯、乙酸异戊酯、异戊醇、2-苯乙醇和芳樟醇等7种挥发性物质,对其气味活性值(oav)进行了定量分析。在C12和EC1118发酵的混合果汁中,辛酸乙酯(果味、花香和菠萝味)的oav最高。酿酒酵母EC1118的总挥发物含量最高(359.23 mg/L),总酯含量也较高(95.50 mg/L)。此外,酿酒葡萄球菌菌株EC1118可以在葡萄酒中提供更高含量的水果香气,这是由oav计算得出的。所有小组成员对葡萄酒的感官分析都可以接受,他们将葡萄酒的颜色、清晰度、味道、风味和总体接受度评分从“稍微喜欢”到“中等喜欢”。
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