Yeasts Isolated from Traditional Brem Bali Show Stress Tolerance Phenotype against Fermentation-Related Stresses

IF 0.8 Q3 MULTIDISCIPLINARY SCIENCES Makara Journal of Science Pub Date : 2021-01-01 DOI:10.7454/MSS.V25I1.1209
Audria Bayu Lenka, R. Astuti, S. Listiyowati
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引用次数: 2

Abstract

The search for microbes, primarily yeasts with unique characters such as the tolerance against fermentation-relatedstresses, is gaining significant interest nowadays. Traditionally made alcoholic beverages can be used as sources for such yeasts, given that during fermentation and storage, microbes may develop stress tolerance responses leading to naturally stress-tolerant yeast strains. In this study, we used an alcoholic beverage, that is, Brem Bali, as the source of potential yeast isolates. We isolated nine yeast isolates from two traditional Brem Bali products. All isolates showed tolerance against high glucose stress (40–50%) and sensitivity against high-temperature stress (37–50 °C). Notably, isolate BT5 showed tolerance phenotype against ethanol stress (up to 12.5%). Notably, the ethanol stress tolerance phenotype shown by isolate BT5 was unlikely correlated to the ability of the isolates in combating other stresses. Based on the internal transcribed spacer sequence, isolates BT2, BT5, and BT6 shared high similarity with Wickerhamomyces anomalus (98%). Further approaches may be needed to clarify the identity of these isolates. Based on our study, isolate BT5 bears potential as a fermentation agent based on its ability to combat high glucose and ethanol stresses.
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从传统巴利酵母中分离的酵母对发酵相关的胁迫表现出耐受性表型
寻找微生物,主要是具有独特特性的酵母,如对发酵相关压力的耐受性,目前正引起人们极大的兴趣。传统制造的酒精饮料可以作为这种酵母的来源,因为在发酵和储存过程中,微生物可能会产生耐受性反应,从而产生自然的耐受性酵母菌株。在这项研究中,我们使用一种酒精饮料,即Brem Bali,作为潜在酵母菌分离物的来源。从两种传统的Brem Bali产品中分离出9株酵母菌。所有分离株均表现出高糖耐受性(40-50%)和高温敏感性(37-50℃)。值得注意的是,分离物BT5对乙醇胁迫表现出耐受性表型(高达12.5%)。值得注意的是,分离物BT5显示的乙醇耐受性表型不太可能与分离物抵抗其他胁迫的能力相关。根据内部转录的间隔序列,分离物BT2、BT5和BT6与Wickerhamomyces anomalus具有高度的相似性(98%)。可能需要进一步的方法来澄清这些分离株的身份。根据我们的研究,分离BT5具有作为发酵剂的潜力,基于其对抗高葡萄糖和乙醇应激的能力。
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来源期刊
Makara Journal of Science
Makara Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.30
自引率
20.00%
发文量
24
审稿时长
24 weeks
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