Study on phytochemical, antibacterial and antioxidant properties of Genitiana kurroo Royle

Aditi Sharma, Mamta Devi Sharma, A. Kumar Sinha, P. Mishra, S. Kulshrestha
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Abstract

Gentiana kurroo Royle is an endangered bitter medicinal plant of the Indian subcontinent region. This medicinal plant mainly grows in Kashmir, Himachal Pradesh, and North-west Himalayas. Native people frequently use the medicinal plant’s root and rhizome for various local remedies. The leaf and root samples of the plant were studied for their phytochemicals screening and antibacterial and antioxidant properties. The methanolic root extract as a comparison to methanolic leaf extract was detected with high concentration of phytochemicals like alkaloids, flavonoids, saponins, phenol, glycosides whereas, other phytochemicals like carbohydrates, tannins, and terpenoids showed equal concentration in methanolic root and leaf extracts. In antibacterial study, the methanolic root extract was found to exhibit a maximum zone of inhibition (33 ± 1) against E.fecalis and minimum zone (17 ± 2) against E. coli. The methanolic leaf extract showed a maximum zone of inhibition (31 ± 1) against E. fecalis and a minimum zone (18 ± 2) against K. pneumonia. The antioxidant activity of G.kurroo revealed that the methanolic extracts of root as compared to the methanolic extract of leaves showed comparatively high antioxidant activity and this is due to the presence of high phenol and flavonoid content.
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龙胆的植物化学、抗菌和抗氧化性能研究
龙胆是印度次大陆地区一种濒临灭绝的苦药用植物。这种药用植物主要生长在克什米尔、喜马偕尔邦和西北喜马拉雅地区。当地人经常使用药用植物的根和根茎来进行各种当地的治疗。对该植物的叶和根样品进行了植物化学物质筛选和抗菌抗氧化性能的研究。与乙醇叶提取物相比,甲醇根提取物中生物碱、黄酮类、皂苷、酚、苷类等植物化学物质含量较高,而其他植物化学物质如碳水化合物、单宁、萜类等在甲醇根提取物和叶提取物中含量相当。在抗菌实验中,发现甲醇根提取物对粪肠杆菌的抑制区最大(33±1),对大肠杆菌的抑制区最小(17±2)。甲醇叶提取物对粪肠杆菌的最大抑制区(31±1),对肺炎克雷伯菌的最小抑制区(18±2)。结果表明,根甲醇提取物的抗氧化活性高于叶甲醇提取物,这是由于根甲醇提取物中酚和类黄酮含量较高所致。
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