Molecular Characterization of Virus Infecting Momordica charantia Linn and the Application of Trichoderma viride as Biocontrol Agent in Baccocco Cross River State, Nigeria

Abstract

Momordica charantia Linn, commonly called bitter-melon or ampalaya, is a vigorous, tendril-bearing, frost tender, annual vine of the cucurbitacae family reported to play significant role in health and nutrition. Chlorotic spot symptoms were observed on this plant during a visit to some Gardens in Baccocco Cross River State, Nigeria in 2021. The aim of this study was to identify the virus infecting this plant and investigate the ability of Trichoderma viride to control the virus. Infected leaf samples of M. charantia were collected and maintained on young seedlings of cucumber through mechanical inoculation which was further used to test the ability of Trichoderma viride as biocontrol agent, the sample was further tested against RT-PCR. Result obtained from Gene sequence analysis revealed 87 % nucleotide sequence identity with Morroccan watermelon mosaic virus. This is the first report of MWMV infecting M. charantia in Nigeria. The result further showed that Trichoderma viride was very effective in the control of virus the pathogen.