Detection of matrix metalloproteinase gene expression by reverse transcription-polymerase chain reaction in human hepatocellular carcinoma

International Hepatology Communications Pub Date : 1996-11-01 DOI:10.1016/S0928-4346(96)00324-6

Iwata Ozaki, Kyosuke Yamamoto, Toshihiko Mizuta, Yoichi Setoguchi, Fumitaka Morito, Takahiro Sakai

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引用次数: 7

Abstract

Matrix metalloproteinase (MMP) has been reported to play an important role in the process of tumor development and metastasis through the alterations of cell-cell and/or cell-extracellular matrix interactions. We studied the gene expression of matrix metalloproteinase MMP-2 and MMP-7, and also TIMP-1, one of the inhibitors of metalloproteinase, in the tissue of human hepatocellular carcinoma by using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) method. MMP-2 and MMP-7 have been expressed in advanced hepatocellular carcinomas as well as in non-cancerous, chronically diseased liver tissue, and the expression levels of these MMPs in human hepatocellular carcinoma (HCC) tissues were greater than that of non-cancerous tissues. This data suggests that MMP-2 and MMP-7 may be involved in the process of the development, invasion and metastasis of HCCs.

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逆转录-聚合酶链反应检测肝癌组织基质金属蛋白酶基因表达

据报道，基质金属蛋白酶(MMP)通过改变细胞间和/或细胞间和细胞外基质的相互作用，在肿瘤的发展和转移过程中发挥重要作用。采用半定量逆转录聚合酶链式反应(RT-PCR)方法，研究了基质金属蛋白酶MMP-2、MMP-7及金属蛋白酶抑制剂TIMP-1在人肝癌组织中的基因表达。MMP-2和MMP-7在晚期肝细胞癌以及非癌性慢性病变肝组织中均有表达，且这些MMPs在人肝细胞癌(HCC)组织中的表达水平高于非癌性组织。这些数据提示MMP-2和MMP-7可能参与了hcc的发生、侵袭和转移过程。

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International Hepatology Communications

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