Responses of Antioxidants Enzymes to Oxidative Stress in the Floral Species Drimia Maritime

Khairallah Yara R, Houri Tarek, Osta Bilal, Romanos Danny M, Haddad Georges R
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Abstract

In natural habitats, plants that encounter adverse growth conditions are preserved in natural reserves where rare or endangered species, such the rare plant species “Drimia maritima” are protected. However, abiotic stress, generated by air pollution near Bentael natural reserve Lebanon, where ‘Drimia maritima” is widespread, is suspected to induce alteration in the biochemical status of the plant. The antioxidant potential was determined by measuring the activities variations of five different enzymes related to stress: Catalase (CAT), Superoxide Dismutase (SOD), Ascorbate peroxidase (APX), Guaiacol peroxidase (POX), and Glutathione reductase (GR). The study was conducted in Bentael natural reserve that was exposed to road works on its borders, within three various locations: location 1 directly localized on the road (P1), location 2, opposite to P1 (P2) and a control location in the middle of the reserve (Ctrl). All enzymes showed higher activities in P1 than in the other sites with proportional correlations for all the studied sites. The resulting outcome demonstrate that alteration in enzymes activities are significant element in plant responses to environmental derivatives abiotic stresses which appraise the danger of landscape activities on conserved areas’ wild floral species.
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抗氧化酶对花植物水杨花氧化应激的响应
在自然生境中,遇到不利生长条件的植物被保存在珍稀或濒危物种的自然保护区中,如珍稀植物“水草”。然而,由黎巴嫩本塔尔自然保护区附近的空气污染产生的非生物胁迫被怀疑会导致该植物生化状态的改变。通过测定过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(POX)和谷胱甘肽还原酶(GR)等5种不同应激相关酶的活性变化来测定抗氧化潜力。这项研究是在Bentael自然保护区进行的,该保护区在其边界上有三个不同的位置:位置1直接位于道路上(P1),位置2与P1相反(P2),以及位于保护区中间的控制位置(Ctrl)。所有酶在P1位点的活性均高于其他位点,并呈比例相关。结果表明,酶活性的变化是植物对环境衍生非生物胁迫响应的重要因素,可用于评价景观活动对保护区野生花卉物种的危害。
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