{"title":"Label-Free Intravital Imaging of Cortical Myelin in Mouse Brain by Third-Harmonic Generation Microscopy","authors":"Michael J. Redlich, Hyungsik Lim","doi":"10.1364/boda.2019.jt4a.9","DOIUrl":null,"url":null,"abstract":"We demonstrate label-free intravital imaging of the myelinated fibers in the cerebral cortex of mouse by third-harmonic generation microscopy. Using an optical parametric oscillator as the excitation source, the depth of 250 pm is achieved.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"346 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1364/boda.2019.jt4a.9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
We demonstrate label-free intravital imaging of the myelinated fibers in the cerebral cortex of mouse by third-harmonic generation microscopy. Using an optical parametric oscillator as the excitation source, the depth of 250 pm is achieved.
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