Expression of Heavy Chain Peptide Gamma of Mouse Anticreatine Kinase IgG Antibody in the Yeast Hansenula polymorpha

Abstract

The yeast Hansenula polymorpha was used as a host for the expression of the gamma chain pep tide of the F(subscript ab) fragment of the mouse anticreatine kinase-MIgG antibody. Two expression systems were de signed and assembled based on the promoters of the highly expressed enzymes, formate dehydrogenase (FMD) and methanol oxidase MOX of H. polymorpha. Two expression plasmid vectors, pGAGU and pHAAI, with FMD and MOX promoters, respectively, were constructed and transformed into H. polymorpha LR9. The expression of the gamma chain gene yielded about 400mg/L and 550mg/L, representing 7% and 10% of the total cell protein under the control of FMD and MOX promoters, respectively. The prepro-alpha was in completely processed and most of the gamma chain peptide accumulated within the cells, especially under the control of the FMD promoter.