The synergism of hot water pretreatment and enzymatic hydrolysis in depolymerization of lignocellulosic content of palm kernel cake

Q2 Chemical Engineering Journal of Molecular Catalysis B-enzymatic Pub Date : 2016-12-01 DOI:10.1016/j.molcatb.2016.09.004

Shuofu Mi , Hongqiang Li , Shuying Li , Yejun Han

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引用次数: 6

Abstract

Palm kernel cake (PKC), mainly composed of mannan, lignin and protein, is abundant renewable resource with commercial value. To develop clean and efficient way for PKC refinery, the method based on the synergism of hot water pretreatment (HWP), steam pretreatment (SP) and enzymatic hydrolysis were developed. HWP of 180 °C, 20 min and SP of 121 °C, 20 min showed similar performance for sugar release from PKC. The main saccharides produced from PKC by HWP and SP were mannose and manno-oligosaccharides, while no furfural formed. The surface structure analyzed by SEM showed that HWP enhanced the microporosity of PKC, and the accessibility of which was increased thereafter. When HWP pretreated PKC was further hydrolyzed with enzyme cocktail (cellulase, xylanase, endo-mannanase), 45% of PKC was solubilized compared with the control. The manno-oligosaccharides produced by HWP and SP were converted to mannose and mannobiose by endo-mannanase. The results suggested that both HWP and SP promote enzymatic hydrolysis of PKC by releasing oligosaccharides and enhancing microporosity, and the synergism of which was effective for PKC decomposition.

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热水预处理和酶解对棕榈仁饼木质纤维素含量解聚的协同作用

棕榈仁饼主要由甘露聚糖、木质素和蛋白质组成，是一种丰富的可再生资源，具有商业价值。为开发清洁高效的PKC炼化途径，研究了热水预处理(HWP)、蒸汽预处理(SP)和酶解协同作用的方法。180°C, 20 min的HWP和121°C, 20 min的SP对PKC的糖释放效果相似。采用HWP和SP法制备PKC的主要糖类为甘露糖和低甘露糖，未形成糠醛。SEM分析表明，HWP增强了PKC的微孔隙度，提高了PKC的可达性。用混合酶(纤维素酶、木聚糖酶、甘露聚糖酶)进一步水解HWP预处理的PKC，与对照相比，PKC的溶解率为45%。通过内切甘露聚糖酶将HWP和SP生产的甘露寡糖转化为甘露糖和甘露糖糖。结果表明，HWP和SP均通过释放低聚糖和增加微孔隙度来促进PKC酶解，两者的协同作用对PKC的分解是有效的。

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来源期刊

Journal of Molecular Catalysis B-enzymatic 生物-生化与分子生物学

CiteScore

2.58

自引率

0.00%

发文量

审稿时长

3.4 months

期刊介绍： Journal of Molecular Catalysis B: Enzymatic is an international forum for researchers and product developers in the applications of whole-cell and cell-free enzymes as catalysts in organic synthesis. Emphasis is on mechanistic and synthetic aspects of the biocatalytic transformation. Papers should report novel and significant advances in one or more of the following topics; Applied and fundamental studies of enzymes used for biocatalysis; Industrial applications of enzymatic processes, e.g. in fine chemical synthesis; Chemo-, regio- and enantioselective transformations; Screening for biocatalysts; Integration of biocatalytic and chemical steps in organic syntheses; Novel biocatalysts, e.g. enzymes from extremophiles and catalytic antibodies; Enzyme immobilization and stabilization, particularly in non-conventional media; Bioprocess engineering aspects, e.g. membrane bioreactors; Improvement of catalytic performance of enzymes, e.g. by protein engineering or chemical modification; Structural studies, including computer simulation, relating to substrate specificity and reaction selectivity; Biomimetic studies related to enzymatic transformations.