Gene Expression of Bovine Dental Pulp Cells In Vitro during Odontoblast-like Cell Differentiation

S. Fujiwara, K. Imai, K. Sanada
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引用次数: 1

Abstract

The expression of developmental stage-specific genes osteocalcin, alkaline phosphatase, thrombospondin (Tsp), transforming growth factor (Tgf) -β, fibroblast growth factor (Fgf), activin (Act) during pulp cell differentiation into odontoblast-like cell was examined using primary cultured pulp cells isolated from bovine adults teeth. The cells slowly became multilayered and clustered to form many nodules. RTPCR amplification demonstrated that Tgf-β1 and Fgf-2 expression were increased whereas osteocalcin, alkaline phosphatase, Tsp-1, and Act-βB expression had declined in relation to cellular differentiation. Mineralization in the nodules was confirmed by Alizarin red and calcein blue staining. These results suggest a role of up-regulation of Tgf-β1 and Fgf-2, and down regulation of osteogenic cell differentiation markers including alkaline phosphatase, Tsp-1 and osteocalcin in the mineralization in bovine dental pulp cells.
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牛牙髓细胞在成牙细胞样细胞分化过程中的基因表达
采用原代培养的牛成年牙髓细胞,检测牙髓细胞向成牙细胞样细胞分化过程中骨钙素、碱性磷酸酶、血小板反应蛋白(Tsp)、转化生长因子(Tgf) -β、成纤维细胞生长因子(Fgf)、激活素(Act)等发育阶段特异性基因的表达。细胞慢慢地变成多层并聚集形成许多结节。RTPCR扩增结果显示,Tgf-β1、Fgf-2表达随细胞分化而升高,骨钙素、碱性磷酸酶、Tsp-1、Act-βB表达随细胞分化而降低。茜素红和钙黄蛋白蓝染色证实了结核中的矿化。上述结果提示,Tgf-β1和Fgf-2的上调,碱性磷酸酶、Tsp-1和骨钙素等成骨细胞分化标志物的下调在牛牙髓细胞矿化中的作用。
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