{"title":"Nucleolar fine structure and RNA synthesis in bovine oocytes from antral follicles","authors":"N. Crozet, J. Kanka, J. Motlík, J. Fulka","doi":"10.1002/MRD.1120140108","DOIUrl":null,"url":null,"abstract":"Nucleolar ultrastructural changes occurring in vivo in bovine oocytes during follicular growth were analyzed by electron microscopy. The rates of in vitro incorporation of 3H-uridine by oocytes of the same size class were evaluated by autoradiography. \n \n \n \nOne to two large fibrillogranular, vacuolated nucleoli were present in oocytes from small to medium antral follicles 0.5–3 mm in diameter. These oocytes showed intense hnRNA and rRNA synthesis. The homogeneous, agranular nucleoli in oocytes from follicles 3–4 mm in diameter were composed of a compact fibrillar material. This morphological change was accompanied by an impairment of nucleolar transcriptional activity as well as by a decrease in hnRNA synthesis.","PeriodicalId":12668,"journal":{"name":"Gamete Research","volume":"71 1","pages":"65-73"},"PeriodicalIF":0.0000,"publicationDate":"1986-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"130","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gamete Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/MRD.1120140108","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 130
Abstract
Nucleolar ultrastructural changes occurring in vivo in bovine oocytes during follicular growth were analyzed by electron microscopy. The rates of in vitro incorporation of 3H-uridine by oocytes of the same size class were evaluated by autoradiography.
One to two large fibrillogranular, vacuolated nucleoli were present in oocytes from small to medium antral follicles 0.5–3 mm in diameter. These oocytes showed intense hnRNA and rRNA synthesis. The homogeneous, agranular nucleoli in oocytes from follicles 3–4 mm in diameter were composed of a compact fibrillar material. This morphological change was accompanied by an impairment of nucleolar transcriptional activity as well as by a decrease in hnRNA synthesis.
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