{"title":"UKURAN STOMATA PAKCOY (Brassica rapa L.) HASIL RENDAMAN KOLKISIN SEBAGAI SUMBER BELAJAR BIOLOGI","authors":"Nurdiah Hasana, S. Sarno, L. Hanum","doi":"10.32502/dikbio.v6i2.4430","DOIUrl":null,"url":null,"abstract":"Anatomi tumbuhan adalah mata kuliah wajib yang harus dipelajari oleh mahasiswa Program Studi Biologi dan Pendidikan Biologi. Salah satu materi yang dibahas dalam anatomi tumbuhan adalah stomata pada daun. Peningkatan ukuran stomata yang diberi kolkisin dapat digunakan sebagai sumber belajar yang memudahkan mahasiswa saat melakukan pengamatan. Sumber belajar tentang stomata dapat menggunakan gambar dari penelitian eksperimen dengan memberikan perendaman biji (benih) pakcoy terlebih dahulu menggunakan kolkisin dengan konsentrasi K0 (0 mg/L), K1(250 mg/L), K2 (500 mg/L), dan K3 (750 mg/L). Pengamatan stomata dilakukan ketika pakcoy berumur 42 hari menggunakan metode replikasi. Stomata diamati menggunakan mikroskop binokuler olympus dengan perbesaran 40x10 yang dihubungkan dengan kamera mikroskop optilab advance by Miconos untuk pengambilan gambar. Pengukuran panjang dan lebar stomata menggunakan software image raster 3. Hasil penelitian menunjukkan ukuran stomata pakcoy (Brassica rapa L.) semakin besar seiring dengan semakin tinggi konsentrasi kolkisin yang digunakan yang ditandai dengan pertambahan ukuran panjang dan lebar stomata. Ukuran stomata yang besar yang disajikan sebagai sumber belajar berupa gambar stomata pakcoy memudahkan mahasiswa dalam mengamati struktur dan bentuk stomata dan menentukan bagian-bagian stomata yang terdiri atas celah stoma, sel penutup dan sel tetangga. Plant anatomy is a compulsory subject that must be studied by students of Biology and Biology Education Study Programs. One of the materials discussed in plant anatomy is the stomaa on the leaves. Increasing the size of stomata given colchicine can be used as a learning resource that makes it easier for students to make observations. The learning resource about stomata could use pictures from experimental research by soaking pakcoy seeds first using colchicine with concentrations of K0 (0 mg/L), K1(250 mg/L), K2 (500 mg/L), and K3 (750 mg/L). The stomata observation was carried out when the pakcoy were 42 days old using the replication method. The stomata were observed using an Olympus binocular microscope with a magnification of 40x10 connected to an optilab advance by Miconos microscope camera for image capture. The stomata length and width were measured using image raster 3 software. The results showed that pakcoy (Brassica rapa L.) stomata size was getting bigger with the higher concentration of colchicine used which was indicated by the increase in the length and width of the stomata. The large size of stomata which was presented as a learning resource in the form of pictures of pakcoy stomata made it easier for students to observe the structure and shape of the stomata and determine the parts of the stomata which consisted of stoma slit, guard cells and neighboring cells.","PeriodicalId":31104,"journal":{"name":"Bioeksperimen Jurnal Penelitian Biologi","volume":"77 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioeksperimen Jurnal Penelitian Biologi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32502/dikbio.v6i2.4430","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Anatomi tumbuhan adalah mata kuliah wajib yang harus dipelajari oleh mahasiswa Program Studi Biologi dan Pendidikan Biologi. Salah satu materi yang dibahas dalam anatomi tumbuhan adalah stomata pada daun. Peningkatan ukuran stomata yang diberi kolkisin dapat digunakan sebagai sumber belajar yang memudahkan mahasiswa saat melakukan pengamatan. Sumber belajar tentang stomata dapat menggunakan gambar dari penelitian eksperimen dengan memberikan perendaman biji (benih) pakcoy terlebih dahulu menggunakan kolkisin dengan konsentrasi K0 (0 mg/L), K1(250 mg/L), K2 (500 mg/L), dan K3 (750 mg/L). Pengamatan stomata dilakukan ketika pakcoy berumur 42 hari menggunakan metode replikasi. Stomata diamati menggunakan mikroskop binokuler olympus dengan perbesaran 40x10 yang dihubungkan dengan kamera mikroskop optilab advance by Miconos untuk pengambilan gambar. Pengukuran panjang dan lebar stomata menggunakan software image raster 3. Hasil penelitian menunjukkan ukuran stomata pakcoy (Brassica rapa L.) semakin besar seiring dengan semakin tinggi konsentrasi kolkisin yang digunakan yang ditandai dengan pertambahan ukuran panjang dan lebar stomata. Ukuran stomata yang besar yang disajikan sebagai sumber belajar berupa gambar stomata pakcoy memudahkan mahasiswa dalam mengamati struktur dan bentuk stomata dan menentukan bagian-bagian stomata yang terdiri atas celah stoma, sel penutup dan sel tetangga. Plant anatomy is a compulsory subject that must be studied by students of Biology and Biology Education Study Programs. One of the materials discussed in plant anatomy is the stomaa on the leaves. Increasing the size of stomata given colchicine can be used as a learning resource that makes it easier for students to make observations. The learning resource about stomata could use pictures from experimental research by soaking pakcoy seeds first using colchicine with concentrations of K0 (0 mg/L), K1(250 mg/L), K2 (500 mg/L), and K3 (750 mg/L). The stomata observation was carried out when the pakcoy were 42 days old using the replication method. The stomata were observed using an Olympus binocular microscope with a magnification of 40x10 connected to an optilab advance by Miconos microscope camera for image capture. The stomata length and width were measured using image raster 3 software. The results showed that pakcoy (Brassica rapa L.) stomata size was getting bigger with the higher concentration of colchicine used which was indicated by the increase in the length and width of the stomata. The large size of stomata which was presented as a learning resource in the form of pictures of pakcoy stomata made it easier for students to observe the structure and shape of the stomata and determine the parts of the stomata which consisted of stoma slit, guard cells and neighboring cells.