Micellar high performance liquid chromatographic determination of flunixin meglumine in bulk, pharmaceutical dosage forms, bovine liver and kidney

Abstract

A simple, sensitive and rapid liquid chromatographic method was developed and validated for the analysis of flunixin meglumine (flunixin-M) in bulk, pharmaceutical dosage forms, bovine liver and kidney. Analytical separation was performed in less than 4 min using a C₁₈ column with UV detection at 284 nm. A micellar solution composed of 0.15 M sodium dodecyl sulphate, 8% n-butanol and 0.3% triethylamine in 0.02 M phosphoric acid buffered at pH 7.0 was used as the mobile phase. The method was fully validated in accordance with the International Conference on Harmonization (ICH) guidelines. The limit of detection and the limit of quantitation were 0.02 and 0.06 μg mL⁻¹, respectively. The recoveries obtained were in range of 95.58–106.94% for bovine liver and kidney. High extraction efficiency was obtained without matrix interference in the extraction process and in the subsequent chromatographic determination. The method showed good repeatability, linearity and sensitivity according to the evaluation of the validation parameters.