Construction of an All-in-one Double-conditional shRNA Expression Vector

Abstract

: Gene silencing by RNA interference （ RNAi ） is widely used for assessing gene function. field the discovery that plasmid-based RNAi for synthetic small interfering RNA in vitro and in vivo we confirmed functional recombination of the floxed sequence of loxP-like TATA-lox by analysing restriction enzyme-digested fragments. This all-in-one double-conditional shRNA expression vector will be useful for reversible in vitro and in vivo knockdown of target gene expression, in target cells via promoter-specific expression of NCre, and at specific times by tetracycline application.