Influence of gelatin modification on enzymatically-gellable pectin-gelatin hydrogel properties for soft tissue engineering applications

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Bioactive and Compatible Polymers Pub Date : 2022-08-26 DOI:10.1177/08839115221119210

Asal Ebrahimzadeh, Elnaz Khanalizadeh, Shahla Khodabakhshaghdam, D. Kazemi, Ali Baradar Khoshfetrat

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Abstract

Injectable in situ-forming hydrogels appears to be a promising approach for tissue engineering applications. In this study, the effect of phenol moiety (Ph) addition to gelatin in enzymatically-gellable modified pectin hydrogel (Pec-Ph) was studied. Addition of gelatin-Ph to Pec-Ph (Pec-Ph/Gel-Ph) altered the physical properties of Pec-Ph-based hydrogels as compared to unmodified gelatin (Pec-Ph/Gel) addition. Swelling ratio and degradation rates of the Pec-Ph/Gel-Ph hydrogel decreased 35% and 50%, respectively, and the elasticity of Pec-Ph/Gel-Ph hydrogel was higher than the Pec-Ph/Gel hydrogels. Scanning electron microscopy images showed that the existence of phenolic groups in gelatin decreased the pore size of Pec-Ph/Gel-Ph hydrogels. Culture of chondrocyte cells in the Pec-Ph/Gel-Ph hydrogels showed more metabolic activity (4×) during a 14-day culture period. Hydrogels subcutaneously implanted in rats could also be identified readily without complete absorption and signs of toxicity or any untoward reactions after 1 month. The work showed the potential of Pec-Ph/Gel-Ph hydrogels as a promising in situ injectable hydrogel for soft tissue engineering applications.

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明胶改性对酶凝胶性果胶-明胶水凝胶性能的影响

可注射的位置形成水凝胶似乎是一个有前途的方法，组织工程应用。本文研究了在酶凝胶改性果胶水凝胶(Pec-Ph)中加入苯酚段(Ph)对明胶性能的影响。与未改性明胶(pecph /Gel)相比，明胶- ph加入到pecph (pecph /Gel)中改变了pecph基水凝胶的物理性质。pecph /Gel- ph水凝胶的溶胀率和降解率分别降低35%和50%，其弹性高于pecph /Gel水凝胶。扫描电镜图像显示，明胶中酚基的存在使peg - ph /Gel-Ph水凝胶的孔径减小。在peg - ph /Gel-Ph水凝胶中培养的软骨细胞在14天的培养期间显示出更高的代谢活性(4倍)。大鼠皮下植入水凝胶，1个月后无完全吸收，无毒性和不良反应迹象。该研究表明，Pec-Ph/Gel-Ph水凝胶是一种有前景的软组织工程原位注射水凝胶。

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来源期刊

Journal of Bioactive and Compatible Polymers 工程技术-材料科学：生物材料

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： The use and importance of biomedical polymers, especially in pharmacology, is growing rapidly. The Journal of Bioactive and Compatible Polymers is a fully peer-reviewed scholarly journal that provides biomedical polymer scientists and researchers with new information on important advances in this field. Examples of specific areas of interest to the journal include: polymeric drugs and drug design; polymeric functionalization and structures related to biological activity or compatibility; natural polymer modification to achieve specific biological activity or compatibility; enzyme modelling by polymers; membranes for biological use; liposome stabilization and cell modeling. This journal is a member of the Committee on Publication Ethics (COPE).