An experimental strategy validated to design cost-effective culture media based on response surface methodology

J. L. Navarrete-Bolaños, M. G. Téllez-Martínez, R. Miranda-López, H. Jiménez-Islas
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引用次数: 11

Abstract

ABSTRACT For any fermentation process, the production cost depends on several factors, such as the genetics of the microorganism, the process condition, and the culture medium composition. In this work, a guideline for the design of cost-efficient culture media using a sequential approach based on response surface methodology is described. The procedure was applied to analyze and optimize a culture medium of registered trademark and a base culture medium obtained as a result of the screening analysis from different culture media used to grow the same strain according to the literature. During the experiments, the procedure quantitatively identified an appropriate array of micronutrients to obtain a significant yield and find a minimum number of culture medium ingredients without limiting the process efficiency. The resultant culture medium showed an efficiency that compares favorably with the registered trademark medium at a 95% lower cost as well as reduced the number of ingredients in the base culture medium by 60% without limiting the process efficiency. These results demonstrated that, aside from satisfying the qualitative requirements, an optimum quantity of each constituent is needed to obtain a cost-effective culture medium. Study process variables for optimized culture medium and scaling-up production for the optimal values are desirable.
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一种基于响应面法设计高性价比培养基的实验策略得到了验证
对于任何发酵工艺,生产成本取决于几个因素,如微生物的遗传、工艺条件和培养基组成。在这项工作中,描述了使用基于响应面方法的顺序方法设计具有成本效益的培养基的指导方针。根据文献对同一菌种不同培养基筛选分析得到的注册商标培养基和基础培养基进行了分析和优化。在实验过程中,该程序定量地确定了适当的微量营养素阵列,以获得显著的产量,并在不限制工艺效率的情况下找到最少数量的培养基成分。所得培养基的效率比注册商标培养基低95%,并且在不限制工艺效率的情况下,将基础培养基中的成分数量减少了60%。这些结果表明,除了满足质量要求外,每种成分的最佳数量需要获得具有成本效益的培养基。研究最佳培养基的工艺变量,并扩大生产以获得最佳值。
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