Effect of tagitinin C isolated from Tithonia diversifoli (Hemsley) A Gray on migration activity and TGF-β1 levels on keloid fibroblast

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-08-20 DOI:10.19106/jmedsci005103201901

E. Santi, M. Wahyuningsih, A. Budiyanto

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引用次数: 2

Abstract

Keloid is the formation of excessive scar tissue characterized by fibroblast hiperproliferations and collagen deposits that are similar with cancer cells. Tagitinin C is proven can inhibit proliferation and deposition of keloids collagen fibroblast. However, the mechanism of action of tagitinin C in migration activities and TGF-β1 levels of keloid fibroblasts has not been proved, yet. This study aimed to investigate the effects of tagitinin C isolated from Tithonia diversifoli (Hemsley) on migration activity and TGF-β1 expression of keloid fibroblast. This was quasi experimental study with post test only controlled group design using keloid fibroblasts isolated from keloid patients. The migration activity were performed by scratch assay and TGF-β1 levels were measured using an ELISA kits. Isolate tagitinin C was more active inhibit fibroblast keloid migration compare to the control groups (p<0.05) after 48 h incubation. TGF-β1 levels after incubation with isolate tagitinin C was lower then control group (p<0.05). In conclusion, isolate tagitinin C can inhibit migration and reduce TGF-β1 levels on keloid fibroblast

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山楂塔吉丁素C对瘢痕疙瘩成纤维细胞迁移活性及TGF-β1水平的影响

瘢痕疙瘩是一种以成纤维细胞增生和胶原沉积为特征的过度疤痕组织，与癌细胞相似。Tagitinin C被证明可以抑制瘢痕疙瘩胶原成纤维细胞的增殖和沉积。然而，tagitinin C对瘢痕疙瘩成纤维细胞迁移活性及TGF-β1水平的作用机制尚未得到证实。本研究旨在探讨从千叶铁中分离的塔吉丁素C对瘢痕疙瘩成纤维细胞迁移活性及TGF-β1表达的影响。这是一项准实验研究，使用从瘢痕疙瘩患者中分离的瘢痕疙瘩成纤维细胞进行实验后仅对照组设计。采用划痕法检测迁移活性，ELISA试剂盒检测TGF-β1水平。培养48 h后，与对照组相比，分离的他吉宁C对成纤维细胞瘢痕疙瘩迁移的抑制作用更强(p<0.05)。TGF-β1与对照组相比显著降低(p<0.05)。综上所述，分离tagitinin C可抑制瘢痕疙瘩成纤维细胞的迁移，降低TGF-β1水平

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Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)

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