{"title":"Plant Regeneration from Cotyledon Explants of Bittergourd as influenced by Adenine Sulphate and D-Biotin","authors":"J. A. D. C. Kawshalya, T. Seran","doi":"10.4038/jfa.v16i1.5280","DOIUrl":null,"url":null,"abstract":"The first experiment was done to select the most suitable explant to establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP and 0.2 mg l-1 NAA. Cotyledon was the most responsive explant which exhibited quick callus initiation. The second experiment was conducted to determine the effect of cotyledon explants on different MS media containing 1.0 mg l-1 BAP, 0.2 mg l-1 NAA and also various concentrations (0, 20, 40 and 60 mg l-1) of adenine sulphate (AS). The best medium for callus formation was MS medium with 1.0 mg l-1 BAP, 0.2 mg l-1 NAA and 60 mg l-1 AS. Callus derived from the particular medium also showed the best positive response for shoot regeneration after subculturing on MS medium with 2.0 mg l-1 BAP and 0.2 mg l-1 NAA. In the third experiment, D-biotin added into the composition of the culture medium had a beneficial effect on the callogenesis in association with BAP and NAA. Regenerated shoots were multiplied on MS medium with 3.0 mg l-1 BAP. Eventually, in vitro rooting was achieved on MS medium included with 1.0 mg l-1 IBA.","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Emirates Journal of Food and Agriculture","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4038/jfa.v16i1.5280","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
The first experiment was done to select the most suitable explant to establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP and 0.2 mg l-1 NAA. Cotyledon was the most responsive explant which exhibited quick callus initiation. The second experiment was conducted to determine the effect of cotyledon explants on different MS media containing 1.0 mg l-1 BAP, 0.2 mg l-1 NAA and also various concentrations (0, 20, 40 and 60 mg l-1) of adenine sulphate (AS). The best medium for callus formation was MS medium with 1.0 mg l-1 BAP, 0.2 mg l-1 NAA and 60 mg l-1 AS. Callus derived from the particular medium also showed the best positive response for shoot regeneration after subculturing on MS medium with 2.0 mg l-1 BAP and 0.2 mg l-1 NAA. In the third experiment, D-biotin added into the composition of the culture medium had a beneficial effect on the callogenesis in association with BAP and NAA. Regenerated shoots were multiplied on MS medium with 3.0 mg l-1 BAP. Eventually, in vitro rooting was achieved on MS medium included with 1.0 mg l-1 IBA.
期刊介绍:
The "Emirates Journal of Food and Agriculture [EJFA]" is a unique, peer-reviewed Journal of Food and Agriculture publishing basic and applied research articles in the field of agricultural and food sciences by the College of Food and Agriculture, United Arab Emirates University, United Arab Emirates.