Plant Regeneration from Cotyledon Explants of Bittergourd as influenced by Adenine Sulphate and D-Biotin

IF 0.7 4区 农林科学 Q3 AGRONOMY Emirates Journal of Food and Agriculture Pub Date : 2023-07-07 DOI:10.4038/jfa.v16i1.5280
J. A. D. C. Kawshalya, T. Seran
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Abstract

The first experiment was done to select the most suitable explant to establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP and 0.2 mg l-1 NAA. Cotyledon was the most responsive explant which exhibited quick callus initiation. The second experiment was conducted to determine the effect of cotyledon explants on different MS media containing 1.0 mg l-1 BAP, 0.2 mg l-1 NAA and also various concentrations (0, 20, 40 and 60 mg l-1) of adenine sulphate (AS). The best medium for callus formation was MS medium with 1.0 mg l-1 BAP, 0.2 mg l-1 NAA and 60 mg l-1 AS. Callus derived from the particular medium also showed the best positive response for shoot regeneration after subculturing on MS medium with 2.0 mg l-1 BAP and 0.2 mg l-1 NAA. In the third experiment, D-biotin added into the composition of the culture medium had a beneficial effect on the callogenesis in association with BAP and NAA. Regenerated shoots were multiplied on MS medium with 3.0 mg l-1 BAP. Eventually, in vitro rooting was achieved on MS medium included with 1.0 mg l-1 IBA.
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硫酸腺嘌呤和d -生物素对苦瓜子叶外植体植株再生的影响
第一次试验选择最适宜的外植体建立芽再生培养。从离体苗中取出4个不同的外植体(子叶、子叶节、叶片和下胚轴),在含1.0 mg l-1 BAP和0.2 mg l-1 NAA的MS培养基上培养。子叶外植体对愈伤组织形成反应最快,愈伤组织形成速度最快。第二项试验研究了子叶外植体在含1.0 mg l-1 BAP、0.2 mg l-1 NAA和不同浓度(0、20、40和60 mg l-1)硫酸腺嘌呤(AS)的MS培养基上的生长效果。愈伤组织形成的最佳培养基为MS + 1.0 mg l-1 BAP + 0.2 mg l-1 NAA + 60 mg l-1 AS的培养基。在含2.0 mg l-1 BAP和0.2 mg l-1 NAA的MS培养基上继代后,愈伤组织再生效果最好。在第三个实验中,在培养基中添加d -生物素,与BAP和NAA共同作用,对骨痂形成有促进作用。再生芽在含3.0 mg l-1 BAP的MS培养基上增殖。最终,在含1.0 mg l-1 IBA的MS培养基上实现离体生根。
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来源期刊
Emirates Journal of Food and Agriculture
Emirates Journal of Food and Agriculture AGRONOMYFOOD SCIENCE & TECHNOLOGY&nb-FOOD SCIENCE & TECHNOLOGY
CiteScore
1.80
自引率
0.00%
发文量
18
期刊介绍: The "Emirates Journal of Food and Agriculture [EJFA]" is a unique, peer-reviewed Journal of Food and Agriculture publishing basic and applied research articles in the field of agricultural and food sciences by the College of Food and Agriculture, United Arab Emirates University, United Arab Emirates.
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