A new species of Hypsibius (Phylum Tardigrada) from Roan Mountain, Tennessee, U.S.A.

D. Nelson, Karen L. McGlothlin
{"title":"A new species of Hypsibius (Phylum Tardigrada) from Roan Mountain, Tennessee, U.S.A.","authors":"D. Nelson, Karen L. McGlothlin","doi":"10.2307/3226827","DOIUrl":null,"url":null,"abstract":"A new tardigrade species, Hypsibius roanensis, is described from epiphytic mosses and lichens on beech trees on Roan Mountain, Tennessee. It differs from other species of the genus by the presence of a sculptured cuticle with irregular tubercles that increase in size caudally. The buccopharyngeal apparatus consists of a narrow buccal tube, well-developed apophyses, and a spherical pharynx with two granular macroplacoids. Roan Mountain is the highest cross range between the Unaka Mountains and the Blue Ridge Mountains in the Southern Appalachians. The north-facing slope of the mountain is in Carter County, Tennessee; the south-facing slope is in Mitchell County, North Carolina. Tardigrades were first recorded from Roan Mountain by Riggin (1962), who found three species of Macrobiotus at 1,890 m in Tennessee. Maucci (1987) reported 10 species in five genera (Macrobiotus, Diphascon, Minibiotus, Itaquascon, and Milnesium) from mosses collected in the spruce-fir forest of Roan Mountain above 1,829 m. The most extensive study of tardigrades on Roan Mountain was by Nelson (1973, 1975), who examined the ecological distribution of 21 species from epiphytic mosses on beech trees at approximately 1,219 m, 1,524 m, and 1,654 m on both the northand southfacing slopes. Nelson found nine specimens of a new species of Hypsibius (designated Hypsibius \"a\" in Nelson, 1975). However, multiple specimens from a sample were mounted per slide in a non-permanent medium (Turtox CMC9), and the slides were unsuitable for identification after 10 years. McGlothlin (1990) collected again at two of Nelson's sites to determine the long-term stability of tardigrade populations and rediscovered the new species of Hypsibius. The new species is described here. MATERIALS AND METHODS On 8 September 1988, epiphytes (primarily mosses) were collected from beech trees at approximately 1,524 m and 1,654 m on the north-facing slope of Roan Mountain, Carter County, Tennessee (Nelson's stations 5N and 6N). Fifty-six samples were collected, 27 from the 5N station and 29 from 6N. Each sample was placed in a stoppered funnel and soaked in tap water for 24 h to allow the animals to become active and then go into anoxybiosis. The epiphyte was removed and squeezed over the funnel to recover the maximal number of tardigrades. The contents of the funnel were drained into a beaker, and the top layer of water was decanted. Boiling water was added to the beaker to kill the tardigrades. Then the contents of the beaker were rinsed through two sieves, TRANS. AM. MICROSC. SOC., 112(2): 140-144. 1993. ? Copyright, 1993, by the American Microscopical Society, Inc. This content downloaded from 157.55.39.127 on Tue, 28 Jun 2016 06:58:43 UTC All use subject to http://about.jstor.org/terms VOL. 112, NO. 2, APRIL 1993 no. 18 mesh (1 mm) and no. 325 mesh (0.045 mm), simultaneously. The material remaining on the no. 325 sieve was washed into a jar with 95% ethanol. Later, the contents of the jars were examined in a gridded Petri dish under a darkfield stereomicroscope at 25 x. Tardigrades were transferred with an Irwin loop to small vials containing 95% ethanol. Up to 35 tardigrades were mounted per sample. Each specimen was removed with an Irwin loop and placed in Hoyer's mounting medium on a microscope slide and sealed with a coverslip. After the medium had dried completely, the coverslip was ringed with epoxy paint. Identifications were made using an interference phase-contrast compound microscope with oil immersion. Measurements were made with a calibrated ocular micrometer. Drawings were made with a drawing tube and photographs were taken with Kodak T Max? 100 film. The scanning electron micrograph is from Nelson's (1973) original study of Roan Mountain tardigrades.","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"62 1","pages":"140-144"},"PeriodicalIF":0.0000,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"13","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transactions of the American Microscopical Society","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2307/3226827","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13

Abstract

A new tardigrade species, Hypsibius roanensis, is described from epiphytic mosses and lichens on beech trees on Roan Mountain, Tennessee. It differs from other species of the genus by the presence of a sculptured cuticle with irregular tubercles that increase in size caudally. The buccopharyngeal apparatus consists of a narrow buccal tube, well-developed apophyses, and a spherical pharynx with two granular macroplacoids. Roan Mountain is the highest cross range between the Unaka Mountains and the Blue Ridge Mountains in the Southern Appalachians. The north-facing slope of the mountain is in Carter County, Tennessee; the south-facing slope is in Mitchell County, North Carolina. Tardigrades were first recorded from Roan Mountain by Riggin (1962), who found three species of Macrobiotus at 1,890 m in Tennessee. Maucci (1987) reported 10 species in five genera (Macrobiotus, Diphascon, Minibiotus, Itaquascon, and Milnesium) from mosses collected in the spruce-fir forest of Roan Mountain above 1,829 m. The most extensive study of tardigrades on Roan Mountain was by Nelson (1973, 1975), who examined the ecological distribution of 21 species from epiphytic mosses on beech trees at approximately 1,219 m, 1,524 m, and 1,654 m on both the northand southfacing slopes. Nelson found nine specimens of a new species of Hypsibius (designated Hypsibius "a" in Nelson, 1975). However, multiple specimens from a sample were mounted per slide in a non-permanent medium (Turtox CMC9), and the slides were unsuitable for identification after 10 years. McGlothlin (1990) collected again at two of Nelson's sites to determine the long-term stability of tardigrade populations and rediscovered the new species of Hypsibius. The new species is described here. MATERIALS AND METHODS On 8 September 1988, epiphytes (primarily mosses) were collected from beech trees at approximately 1,524 m and 1,654 m on the north-facing slope of Roan Mountain, Carter County, Tennessee (Nelson's stations 5N and 6N). Fifty-six samples were collected, 27 from the 5N station and 29 from 6N. Each sample was placed in a stoppered funnel and soaked in tap water for 24 h to allow the animals to become active and then go into anoxybiosis. The epiphyte was removed and squeezed over the funnel to recover the maximal number of tardigrades. The contents of the funnel were drained into a beaker, and the top layer of water was decanted. Boiling water was added to the beaker to kill the tardigrades. Then the contents of the beaker were rinsed through two sieves, TRANS. AM. MICROSC. SOC., 112(2): 140-144. 1993. ? Copyright, 1993, by the American Microscopical Society, Inc. This content downloaded from 157.55.39.127 on Tue, 28 Jun 2016 06:58:43 UTC All use subject to http://about.jstor.org/terms VOL. 112, NO. 2, APRIL 1993 no. 18 mesh (1 mm) and no. 325 mesh (0.045 mm), simultaneously. The material remaining on the no. 325 sieve was washed into a jar with 95% ethanol. Later, the contents of the jars were examined in a gridded Petri dish under a darkfield stereomicroscope at 25 x. Tardigrades were transferred with an Irwin loop to small vials containing 95% ethanol. Up to 35 tardigrades were mounted per sample. Each specimen was removed with an Irwin loop and placed in Hoyer's mounting medium on a microscope slide and sealed with a coverslip. After the medium had dried completely, the coverslip was ringed with epoxy paint. Identifications were made using an interference phase-contrast compound microscope with oil immersion. Measurements were made with a calibrated ocular micrometer. Drawings were made with a drawing tube and photographs were taken with Kodak T Max? 100 film. The scanning electron micrograph is from Nelson's (1973) original study of Roan Mountain tardigrades.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
标题美国田纳西州罗安山缓步虫门一新种
在美国田纳西州罗安山山毛榉上的附生苔藓和地衣中发现了一种缓步动物新种——罗安山毛榉。它不同于其他种类的属的存在与不规则的结节,在尾端增加大小的雕刻角质层。咽咽器由狭窄的咽管、发育良好的咽突和球形咽及两个颗粒状的大咽片组成。罗安山是阿巴拉契亚山脉南部乌纳卡山脉和蓝岭山脉之间最高的交叉山脉。山的北坡在田纳西州的卡特县;朝南的斜坡位于北卡罗来纳州的米切尔县。缓步动物最早是由Riggin(1962)在Roan山记录的,他在田纳西州1890米的地方发现了3种Macrobiotus。Maucci(1987)报道了在海拔1829米以上的罗安山云杉林中采集的藓类5属(Macrobiotus, Diphascon, Minibiotus, Itaquascon, Milnesium) 10种。对Roan山缓步动物最广泛的研究是由Nelson(1973, 1975)进行的,他研究了21种附生苔藓在山毛榉树上的生态分布,分布高度分别为1219米、1524米和1654米,分别位于北坡和南坡。纳尔逊发现了9个新物种Hypsibius的标本(Nelson在1975年命名为Hypsibius“a”)。然而,在非永久介质(Turtox CMC9)中,每张载玻片都装载了多个样品,并且载玻片在10年后不适合鉴定。McGlothlin(1990)再次在Nelson的两个地点收集数据,以确定缓步动物种群的长期稳定性,并重新发现了新物种Hypsibius。这里描述了新物种。材料与方法1988年9月8日,在田纳西州卡特县Roan山北坡约1524 m和1654 m处(Nelson站5N和6N)采集了山毛榉的附生植物(主要是苔藓)。共采集56份样品,其中5N站27份,6N站29份。每个样本被放置在一个带塞的漏斗中,在自来水中浸泡24小时,使动物变得活跃,然后进入缺氧状态。将附生菌移出并在漏斗上挤压,以恢复最大数量的缓步动物。漏斗的内容物被排到烧杯中,最上层的水被倒出。烧杯中加入沸水以杀死缓步动物。然后,烧杯里的东西通过两个筛子(TRANS)进行冲洗。点。MICROSC。SOC。浙江农业学报,12(2):140-144。1993. ? 版权所有,1993年,美国显微学会,Inc。此内容于2016年6月28日星期二06:58:43 UTC从157.55.39.127下载。1993年4月2日18目(1毫米),无。325目(0.045毫米),同时。残留在上面的材料。325筛子用95%乙醇洗入罐中。随后,在25倍暗场立体显微镜下的格子培养皿中检查罐子中的内容物。用欧文环将缓步动物转移到含有95%乙醇的小瓶中。每个样本最多安装35个缓步动物。每个标本用Irwin loop取出,放在Hoyer的安装介质中,放在显微镜载玻片上,用盖盖密封。在介质完全干燥后,用环氧涂料将盖盖圈起来。采用油浸干涉相衬复合显微镜进行鉴定。用校准过的眼测微计进行测量。图纸是用拔管绘制的,照片是用柯达T Max?100年的电影。扫描电子显微照片来自尼尔森(1973)对罗安山缓步动物的原始研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
A new copepod (Cyclopoida: Clausidiidae) parasitic on mud shrimps in Louisiana. Chaetonotus triacanthus and Heteroxenotrichula texana two new chaetonotid gastrotrichs from the Gulf of Mexico Physalopteroides bahamensis n. sp. (Nematoda: Spiruroidea) from the Cuban Treefrog Osteopilus septentrionalis (Hylidae) from San Salvador Island, Bahamas New Species of Bertrana and Amazonepeira, Orb-Weaving Spiders from the Neotropics (Araneae: Araneidae) Morphology and Stomatogenesis of Mesanophrys pugettensis n. sp. (Scuticociliatida: Orchitophryidae), a Facultative Parasitic Ciliate of the Dungeness Crab, Cancer magister (Crustacea: Decapoda)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1