Proportions of Pathogenic Bacteria Isolated from Door Locks and Working Benches in Clinical Laboratory: A Laboratory Based Study

Abias Anthon Moshi, Ester Colnel Kyara, P. L. Mabula, Emmanuel Charles Uroki, D. Kajeguka, Sixbert I Mkumbaye
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Abstract

Background: Numerous studies have revealed the association of the door handle and contamination of pathogenic bacteria. Door handles of clinical and research laboratories have higher chances of contamination with pathogenic bacteria during closing and opening with contaminated gloves on, or sometimes after visiting the toilets without the use of disinfectant materials. There is limited epidemiological data regarding bacteria cross contamination of door locks of the Clinical laboratory at Kilimanjaro Christian Medical Centre. This study aimed at providing the proportions of bacteria contaminating medical laboratory doors. Methods: A cross section laboratory-based study was conducted and it involved collection of swab samples from doors and working benches in the clinical laboratory. Results: Prevalence of Staphylococcus aureus, Escherichia coli, Coagulase Negative Staphylococcus, Bacillus spp., Pseudomonas aeroginosa and coliforms were (26%, 22%, 18%, 8%, 4% and 4% respectively. Conclusion: This study has reported high proportion of pathogenic bacteria. The results entails that, internal and external environments are responsible for laboratory door contamination.
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临床实验室门锁和工作台分离致病菌比例的实验室研究
背景:大量研究表明门把手与致病菌污染有关。临床和研究实验室的门把手在关闭和打开被污染的手套时,或者有时在没有使用消毒材料的情况下访问厕所后,被致病菌污染的可能性更高。关于乞力马扎罗山基督教医疗中心临床实验室门锁受到细菌交叉污染的流行病学数据有限。本研究旨在提供细菌污染医学实验室门的比例。方法:采用以实验室为基础的横断面研究方法,从临床实验室的门和工作台上采集拭子样本。结果:金黄色葡萄球菌、大肠杆菌、凝固酶阴性葡萄球菌、芽孢杆菌、铜绿假单胞菌和大肠菌群的感染率分别为26%、22%、18%、8%、4%和4%。结论:本研究报告病原菌比例高。结果表明,内部和外部环境对实验室门污染负有责任。
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