The novel primers for mammal species identification-based mitochondrial cytochrome b sequence: implication for reserved wild animals in Thailand and endangered mammal species in Southeast Asia

IF 1.1 4区生物学 Q4 GENETICS & HEREDITY Mitochondrial Dna Part a Pub Date : 2018-01-02 DOI:10.1080/24701394.2016.1238902

Y. Muangkram, W. Wajjwalku, Akira Amano, M. Sukmak

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引用次数: 11

Abstract

Abstract We presented the powerful techniques for species identification using the short amplicon of mitochondrial cytochrome b gene sequence. Two faecal samples and one single hair sample of the Asian tapir were tested using the new cytochrome b primers. The results showed a high sequence similarity with the mainland Asian tapir group. The comparative sequence analysis of the reserved wild mammals in Thailand and the other endangered mammal species from Southeast Asia comprehensibly verified the potential of our novel primers. The forward and reverse primers were 94.2 and 93.2%, respectively, by the average value of the sequence identity among 77 species sequences, and the overall mean distance was 35.9%. This development technique could provide rapid, simple, and reliable tools for species confirmation. Especially, it could recognize the problematic biological specimens contained less DNA material from illegal products and assist with wildlife crime investigation of threatened species and related forensic casework.

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基于线粒体细胞色素b序列的哺乳动物物种鉴定新引物:对泰国保护野生动物和东南亚濒危哺乳动物的启示

摘要本文介绍了利用线粒体细胞色素b基因序列短扩增子进行物种鉴定的有力技术。用新的细胞色素b引物对亚洲貘的两份粪便样本和一份毛发样本进行了检测。结果表明，该基因序列与亚洲貘类群具有较高的相似性。通过对泰国野生哺乳动物和东南亚其他濒危哺乳动物的比较序列分析，全面验证了我们的引物的潜力。77个种序列的序列一致性平均值分别为94.2和93.2%，总体平均距离为35.9%。该技术可为物种鉴定提供快速、简便、可靠的工具。特别是能够识别含有较少非法制品DNA物质的问题生物标本，协助濒危物种的野生动物犯罪调查和相关的法医案件工作。

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来源期刊

Mitochondrial Dna Part a Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.00

自引率

0.00%

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期刊介绍： Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. Manuscripts on cytosolic and extracellular mtDNA, and on dysfunction caused by alterations in mtDNA integrity as well as methodological papers detailing novel approaches for mtDNA manipulation in vitro and in vivo are welcome. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The Journal also considers manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences, as well as papers that discuss the utility of mitochondrial DNA information in medical studies and in human evolutionary biology.