Optimal fabrication condition of bacteriorhodopsin films by electrophoretic sedimentation technique

Junhong Min , Hyun-Goo Choi , Jeong-Woo Choi , Won Hong Lee , Rak Kim
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引用次数: 10

Abstract

The photocurrent of bacteriorhodopsin (bR) films fabricated by electrophoretic sedimentation (EPS) technique was investigated. The fabrication conditions by EPS, the intensity of electric field and exposure time, affected the photoelectric response and topology of bR films. Based on the photoelectric response and surface topology measured by an atomic force microscope (AFM), the optimal electric field for the fabrication of oriented bR film was 20 V/cm. The exposure time of 45 s did not affect the photocurrent of bR film but longer exposures damaged the bR films. The action spectrum of bR films fabricated by EPS technique was well consistent to the absorption spectrum of bR solution.

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电泳沉淀法制备细菌紫质膜的最佳工艺条件
研究了电泳沉淀法(EPS)制备的细菌视紫红质(bR)薄膜的光电流。EPS制备条件、电场强度和曝光时间影响了bR薄膜的光电响应和拓扑结构。基于原子力显微镜(AFM)测量的光电响应和表面拓扑结构,制备定向bR薄膜的最佳电场为20 V/cm。曝光时间为45 s时,bR膜的光电流不受影响,但曝光时间过长会对bR膜造成损伤。EPS法制备的bR膜的作用谱与bR溶液的吸收谱基本一致。
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