{"title":"Uptake and Processing of in vitro Synthesized Mitochondrial Malate Dehydrogenase by Isolated Watermelon Mitochondria","authors":"Christine Gietl, Bertold Hock","doi":"10.1016/S0044-328X(84)80059-8","DOIUrl":null,"url":null,"abstract":"<div><p>Mitochondrial MDH (EC 1.1.1.37) is synthesized in watermelon cotyledons as a cytosolic, higher molecular weight precursor (41 kd). We now show that the precursor produced in a reticulocyte lysate in the presence of watermelon mRNA, is posttranslationally sequestered by a crude mitochondrial fraction or by mitochondria purified on Percoll® gradients. It is processed to a proteinase resistant form corresponding to the mature subunit (38 kd). Optimal incorporations are obtained with organelles from early stages of seedling development. An uptake of the mature MDH dimer to a proteolytically protected form could not be detected.</p></div>","PeriodicalId":23797,"journal":{"name":"Zeitschrift für Pflanzenphysiologie","volume":"114 5","pages":"Pages 393-401"},"PeriodicalIF":0.0000,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80059-8","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift für Pflanzenphysiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0044328X84800598","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Mitochondrial MDH (EC 1.1.1.37) is synthesized in watermelon cotyledons as a cytosolic, higher molecular weight precursor (41 kd). We now show that the precursor produced in a reticulocyte lysate in the presence of watermelon mRNA, is posttranslationally sequestered by a crude mitochondrial fraction or by mitochondria purified on Percoll® gradients. It is processed to a proteinase resistant form corresponding to the mature subunit (38 kd). Optimal incorporations are obtained with organelles from early stages of seedling development. An uptake of the mature MDH dimer to a proteolytically protected form could not be detected.
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