Metal-organic framework hybrid materials of ZIF-8/RGO for immobilization of D-amino acid dehydrogenase

IF 9 2区材料科学 Q1 CHEMISTRY, PHYSICAL Nano Research Pub Date : 2023-07-15 DOI:10.1007/s12274-023-5811-y

Hangbin Lei, Qian Zhang, Xiaoyan Xiang, Liang Jiang, Shiyan Wang, Lingxuan Duan, Shizhen Wang

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Abstract

Immobilization of D-amino acid dehydrogenase (DAADH) by the assembly of peptide linker was studied for the biosynthesis of D-phenylalanine. Hybrid material of zeolitic imidazolate framework-8 (ZIF-8) combined with reduced graphene oxide (RGO) was applied for the immobilization of DAADH from Ureibacillus thermosphaericus. The recovery rate of DAADH/ZIF-8/RGO was 165.6%. DAADH/ZIF-8/RGO remained 53.4% of its initial activity at 50 °C for 10 h while the free enzyme was inactivated. DAADH/ZIF-8/RGO maintained 70.5% activity in hyperalkaline solution with pH 12. Kinetic parameters indicated that DAADH/ZIF-8/RGO had greater affinity of phenylpyruvate as V_max/K_m of DAADH/ZIF-8/RGO was 1.27-fold than free enzyme. After seven recycles, the activity of DAADH/ZIF-8/RGO remained 64.3%. Furthermore, one-step separation and in situ immobilization of DAADH by ZIF-8/RGO/Ni was carried out with 1.5-fold activity enhancement. Combining peptide linker and metal-organic framework (MOF) immobilization, thermostability and activity of the immobilized DAADH were significantly improved.

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ZIF-8/RGO金属-有机骨架杂化材料固定化d -氨基酸脱氢酶

以d -苯丙氨酸为合成原料，研究了肽连接体组装固定d -氨基酸脱氢酶（DAADH）的方法。采用沸石咪唑酸骨架-8 （ZIF-8）与还原氧化石墨烯（RGO）复合的杂化材料，固定化热球脲杆菌DAADH。DAADH/ZIF-8/RGO的回收率为165.6%。当游离酶失活时，DAADH/ZIF-8/RGO在50℃作用10 h仍保持53.4%的初始活性。DAADH/ZIF-8/RGO在pH为12的高碱性溶液中保持70.5%的活性。动力学参数表明，DAADH/ZIF-8/RGO对苯基丙酮酸具有更强的亲和力，其Vmax/Km为游离酶的1.27倍。经过7次循环后，DAADH/ZIF-8/RGO的活性仍为64.3%。ZIF-8/RGO/Ni一步分离原位固定化DAADH，活性提高1.5倍。结合肽连接剂和金属有机骨架（MOF）固定，固定化的DAADH的热稳定性和活性显著提高。

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来源期刊

Nano Research 化学-材料科学：综合

CiteScore

14.30

自引率

11.10%

发文量

2574

审稿时长

1.7 months

期刊介绍： Nano Research is a peer-reviewed, international and interdisciplinary research journal that focuses on all aspects of nanoscience and nanotechnology. It solicits submissions in various topical areas, from basic aspects of nanoscale materials to practical applications. The journal publishes articles on synthesis, characterization, and manipulation of nanomaterials; nanoscale physics, electrical transport, and quantum physics; scanning probe microscopy and spectroscopy; nanofluidics; nanosensors; nanoelectronics and molecular electronics; nano-optics, nano-optoelectronics, and nano-photonics; nanomagnetics; nanobiotechnology and nanomedicine; and nanoscale modeling and simulations. Nano Research offers readers a combination of authoritative and comprehensive Reviews, original cutting-edge research in Communication and Full Paper formats. The journal also prioritizes rapid review to ensure prompt publication.