A Statistical Analysis of Sequence Features within Genes from Neurospora crassa

Stephanie E. Edelmann, Chuck Staben
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引用次数: 111

Abstract

Edelmann, S. E., and Staben, C. 1994. A statistical analysis of features within genes from Neurospora crassa. Experimental Mycology 18, 70-81. We analyzed gene sequences from Neurospora crassa deposited in GenBank or EMBL for GC content, codon usage, intron prevalence, intron length, exon length, translation initiation sites, and mRNA splice sites. Protein coding regions were 59% GC, and noncoding regions were 49% GC. Codon usage was biased, primarily due to a strong preference for C in the final position of the codons. Over 80% of N. crassa protein coding genes had introns, which are typically 60 nucleotides long. Exons varied greatly in length, but were typically much longer than introns. The distribution of nucleotides surrounding translation initiation and intron splice sites was clearly nonrandom. We derived a consensus translation initiation site of CAMMATGGCT, a 5′-intron donor site of G^GTAAGTnnYCnYY, an internal branchpoint of WRCTRACMnnnnnnYY, and a 3′-acceptor site of WACAG^.

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粗神经孢子虫基因序列特征的统计分析
Edelmann, s.e.和Staben, C. 1994。粗神经孢子虫基因特征的统计分析。实验真菌学18,70-81。我们分析了保存在GenBank或EMBL中的粗神经孢子虫的基因序列,包括GC含量、密码子使用、内含子流行率、内含子长度、外显子长度、翻译起始位点和mRNA剪接位点。蛋白质编码区为59% GC,非编码区为49% GC。密码子的使用是有偏见的,主要是由于密码子的最后位置对C的强烈偏好。80%以上的藜草蛋白编码基因含有内含子,内含子长度一般为60个核苷酸。外显子的长度变化很大,但通常比内含子长得多。核苷酸在翻译起始位点和内含子剪接位点周围的分布显然是非随机的。我们得到了CAMMATGGCT的一致翻译起始位点、G^ gtaagtnnynyy的一个5 ' -内含子供体位点、WRCTRACMnnnnnnYY的一个内部分支位点和WACAG^的一个3 ' -受体位点。
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