{"title":"Immortalization of Hypothalamic Gonadotropin-Releasing Hormone Neurons Using Targeted Oncogene Expression in Transgenic Mice","authors":"R. Weiner, S. Moenter","doi":"10.1006/NCMN.1993.1053","DOIUrl":null,"url":null,"abstract":"Abstract Three clonal gonadotropin-releasing hormone (GnRH) neuronal cell lines were derived from a genetically induced tumor in transgenic mice. A transgene was constructed to target expression of simian virus 40 T antigen to GnRH neurons using the promoter/enhancer domains of the cell-specifically expressed GnRH gene. The resulting GT1 cells were characterized by morphology, the expression of neuron-specific genes, expression and processing of GnRH, pulsatile basal secretion of GnRH, release of GnRH in response to depolarization, and regulation of GnRH release by a variety of neurotransmitters and neuromodulators. By all of these criteria, GT1 cells are highly differentiated neuronal cell lines that provide valuable models for studying the cell biology of neuroendocrine cells.","PeriodicalId":100951,"journal":{"name":"Neuroprotocols","volume":"9 1","pages":"184-188"},"PeriodicalIF":0.0000,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuroprotocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1006/NCMN.1993.1053","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract Three clonal gonadotropin-releasing hormone (GnRH) neuronal cell lines were derived from a genetically induced tumor in transgenic mice. A transgene was constructed to target expression of simian virus 40 T antigen to GnRH neurons using the promoter/enhancer domains of the cell-specifically expressed GnRH gene. The resulting GT1 cells were characterized by morphology, the expression of neuron-specific genes, expression and processing of GnRH, pulsatile basal secretion of GnRH, release of GnRH in response to depolarization, and regulation of GnRH release by a variety of neurotransmitters and neuromodulators. By all of these criteria, GT1 cells are highly differentiated neuronal cell lines that provide valuable models for studying the cell biology of neuroendocrine cells.
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