Biochemical Composition, Toxicity and Bioactivities of the Essential Oil extracted from Coffea arabica L. husks against the Cotton Leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctudiae)

Eman E. Essa, Gawhara M. M. Abu El-Hassan, S. Farag
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Abstract

throughout Therefore, approaches for their a ) analyzed its were screened for insecticidal activity against the larval instars of the littoralis GC/MS analysis revealed that the arabic coffee included 13 compounds of which Oleic acid comprises the largest proportion 65 % which is known for its insecticidal efficacy. Other compounds that have insecticidal activities were also separated; Cyclononasiloxane, octadecamethyl-, n-Hexadecanoic acid, 9,12-Octadecadienoic acid (Z, Z)- and N-Isobutyl-11-(3,4-methylenedioxyphenyl)-2E,4E,10E-undecatrienoic amide. The oil extract was tested against the 4 th larval instars. The results indicated the efficacy of the extract as larvicide with the values of LC 50 and LC 90 equal to 1.8083 and 8.3227 respectively. The application of the oil extract against the larvae resulted in the prolongation of both larval and pupal duration and the decrease of the growth rate, pupation, adult longevity and fecundity. Also, the corrected weight of consumed leaf, consumption index (IC), relative growth rate (RGR), the efficacy of ingested and digested food conversion to body tissue (ECI) and (ECD), respectively and the approximate digestibility (AD) were decreased after the application of the oil extract on the larvae. As a result of the current study, the oil extract from the husk of Coffea arabica revealed its efficacy as both larvicide and deterrent against the 4 th instar larvae of cotton leafworm S. littoralis .
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阿拉比卡咖啡豆皮精油对棉花叶虫的生化成分、毒性及生物活性研究
因此,对其a)分析方法进行了对海蛾幼虫的杀虫活性筛选,GC/MS分析显示,阿拉伯咖啡含有13种化合物,其中油酸所占比例最大,为65%,以其杀虫功效闻名。其他具有杀虫活性的化合物也被分离出来;环壬硅氧烷,十八甲基-,正十六烷酸,9,12-十八二烯酸(Z, Z)-和n-异丁基-11-(3,4-亚甲基二氧苯)- 2e,4E, 10e -十一烯酰胺。对油浸提液对4龄幼虫进行了抑虫试验。结果表明,黄芪提取物的lc50和lc90分别为1.8083和8.3227,具有较好的杀幼虫效果。油浸液对幼虫的处理使幼虫期和蛹期均延长,生长速度、化蛹率、成虫寿命和繁殖力均降低。此外,油浸提物还降低了采食叶的校正重、采食指数(IC)、相对生长率(RGR)、采食和消化食物转化为体组织的效率(ECI)和ECD以及近似消化率(AD)。本研究结果表明,阿拉比卡咖啡皮油提取物对棉花叶虫4龄幼虫具有杀幼虫和抑制作用。
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