{"title":"BIOLOGICAL ACTIVITIES OF Citrulluscolocynthis ETHANOLIC SEED EXTRACT","authors":"W. Mohamed, R. A. Al-Masry, H. Elakkad, A. Osman","doi":"10.21608/zjar.2023.297654","DOIUrl":null,"url":null,"abstract":": The bitter gourd plant Citrullus colocynthis (L.) is a cucurbit that is widespread in desert areas. It has many pharmaceutical, medicinal, and nutritional uses due to its high content of biologically active compounds. In this study, the total phenolic compounds (TPCs) and total flavonoids (TFs) of ethanolic C. colocynthis seed extract were estimated. High-performance liquid chromatography (HPLC) was used to identify the bioactive phenolic compounds. On the other hand, the antioxidant and anticancer activities of this extract were evaluated. The extraction yield from C. colocynthis seed using ethanol was recorded 10 g/100g seed. The total phenol (TPCs) content of the ethanolic extract obtained from C. colocynthis seed flour was 21 mg GAE g -1 dry extract, while the total flavonoid (TFCs) content was 15 mg QE g -1 dry extract. As well as the major phenolic and flavonoid compounds in C. colocynthis seed ethanolic extract, as determined by HPLC. Ellagic acid and cinnamic acid have the highest concentrations (894 and 746 µg/g dry extract, respectively) of all the components. The anticancer activity in vitro of the HCT116 and A-549 human cancer cell lines was evaluated using the MTT assay. The results of the MTT assay demonstrated that the C. colocynthis seed extract inhibited the proliferation of HCT116 and A-549 cells in a dose-dependent manner. C. colocynthis seed extract had an IC 50 against HCT116 and A-549 (454, and 416 µg/mL, respectively). Therefore, C. colocynthis can be used as a source to produce pharmaceutical and medicinal products.","PeriodicalId":23865,"journal":{"name":"Zagazig Journal of Agricultural Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zagazig Journal of Agricultural Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/zjar.2023.297654","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
: The bitter gourd plant Citrullus colocynthis (L.) is a cucurbit that is widespread in desert areas. It has many pharmaceutical, medicinal, and nutritional uses due to its high content of biologically active compounds. In this study, the total phenolic compounds (TPCs) and total flavonoids (TFs) of ethanolic C. colocynthis seed extract were estimated. High-performance liquid chromatography (HPLC) was used to identify the bioactive phenolic compounds. On the other hand, the antioxidant and anticancer activities of this extract were evaluated. The extraction yield from C. colocynthis seed using ethanol was recorded 10 g/100g seed. The total phenol (TPCs) content of the ethanolic extract obtained from C. colocynthis seed flour was 21 mg GAE g -1 dry extract, while the total flavonoid (TFCs) content was 15 mg QE g -1 dry extract. As well as the major phenolic and flavonoid compounds in C. colocynthis seed ethanolic extract, as determined by HPLC. Ellagic acid and cinnamic acid have the highest concentrations (894 and 746 µg/g dry extract, respectively) of all the components. The anticancer activity in vitro of the HCT116 and A-549 human cancer cell lines was evaluated using the MTT assay. The results of the MTT assay demonstrated that the C. colocynthis seed extract inhibited the proliferation of HCT116 and A-549 cells in a dose-dependent manner. C. colocynthis seed extract had an IC 50 against HCT116 and A-549 (454, and 416 µg/mL, respectively). Therefore, C. colocynthis can be used as a source to produce pharmaceutical and medicinal products.
苦瓜属植物Citrullus colocynthis (L.)是一种广泛分布于沙漠地区的瓜类植物。由于其高含量的生物活性化合物,它具有许多制药,药用和营养用途。本研究测定了乙醇茜草籽提取物的总酚类化合物(TPCs)和总黄酮(TFs)含量。采用高效液相色谱法对其生物活性酚类化合物进行了鉴定。另一方面,对该提取物的抗氧化和抗癌活性进行了评价。以乙醇为萃取剂的薏苡仁提取率为10 g/100g。黄酮(tfc)含量为15 mg QE g -1,总酚(tpc)含量为21 mg QE g -1。并通过高效液相色谱法测定了薏苡仁乙醇提取物中的主要酚类和黄酮类化合物。鞣花酸和肉桂酸的浓度最高,分别为894和746µg/g干提取物。采用MTT法评价HCT116和A-549人癌细胞的体外抗癌活性。MTT实验结果表明,绿草籽提取物对HCT116和a -549细胞的增殖有抑制作用,且呈剂量依赖性。对HCT116和A-549的ic50分别为454和416µg/mL。因此,大肠杆菌可作为生产药品和医药产品的原料。