The effects of Stachys lavandulifolia leaves aqueous extract on angiogenesis in chick embryos chorioalantoic membrane and choroids plexus

Abstract

Angiogenesis, formation of new vessels from the primary vessels is involved in physiological condition such as reproduction, wound healing and pathological like Diabetes and tumor. Tumor progression associates with persistent unregulated angiogenesis. This study aimed to investigate the effect of leaves aqueous extract of Stachys lavandulifolia on the angiogenesis in chorioallantoic membrane and choroids plexus of chick embryos. In this experimental study, 30 fertilized eggs of Hy-line race were randomly divided into 5 groups: control group, sham-exposed group and four experimental groups (n=6). In the second day of incubation in sterile condition, a window was opened on eggs. On 8th day, chorioallantoic membrane (CAM) was treated at sham-exposed group with normal saline and at experimental groups with Stachys lavandulifolia aqueous extract (25, 50, 100 mg/kg). On 12th day CAMs, the length and number of vessels were examined and 66 Maryam Tehranipour and Saeide Zafar Balanezhad morphological disorders were studied. Data were calculated and analyzed statistically using t-test and ANOVA. There is no significant difference in the mean number and length of vessels in the control group with the sham-exposed groups. The mean of number and length in all experimental groups shows a meaningful increase in compare with sham group (P<0/05).Morphological abnormalities were not observed in fetuses. According to the results of this research, low doses of Stachys lavandulifolia aqueous extract increase angiogenesis in chorioallantoic membrane and choroids plexus, but it has antiangiogenesis effect in high doses. No significant effect on chick embryo's morphological characteristics observed.