Interaction of Catechu Dye with DNA: Spectroscopic and In Silico Approach

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2016-02-25 DOI:10.1080/15257770.2015.1124997

Hridya Hemachandran, Amrita Anantharaman, R. Priya, G. P. Doss, R. Siva

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引用次数: 9

Abstract

ABSTRACT Catechin, a yellow colored molecule obtained from the wood of Acacia catechu was analyzed for its interaction with synthetic DNA duplexes using spectroscopic analysis. UV-Visible spectroscopic analysis revealed the non-intercalative binding mode. Fourier Transform Infrared spectroscopy (FTIR) analysis expose chemical shift indicated by various vibrational stretches and an increase in the intensity of base stacking was observed by Circular Dichroism (CD), respectively. This inference was further confirmed through nuclear staining technique and also in electrophoretic technique; the dye quenches the fluorescent intensity of ethidium bromide. The result of fluorescence spectroscopy was in concordance with the electrophoretic technique. In addition, the spectroscopic results were in accordance with the molecular docking studies of specific catechin compound from the catechu dye with CT-DNA. This kind of site specificity is a gain in the medicinal field as the drug can be DNA targeted for cancer therapeutics. The present work reveals that catechu dye has a noteworthy application in the field of medical bioscience.

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儿茶染料与DNA的相互作用:光谱和计算机方法

摘要利用光谱分析方法分析了从儿茶树木材中提取的黄色分子儿茶素与合成DNA双链物的相互作用。紫外可见光谱分析显示其非插层结合模式。傅里叶变换红外光谱(FTIR)分析揭示了不同振动拉伸引起的化学位移和圆二色性(CD)观察到基层堆叠强度的增加。核染色技术和电泳技术进一步证实了这一推断;染料使溴化乙锭的荧光强度减弱。荧光光谱分析结果与电泳分析结果一致。此外，光谱结果与从儿茶染料中提取的特定儿茶素化合物与CT-DNA的分子对接研究相一致。这种位点特异性是医学领域的一个新进展，因为这种药物可以作为DNA靶向药物用于癌症治疗。研究表明，儿茶染料在医学生物科学领域具有重要的应用前景。

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Nucleosides, Nucleotides and Nucleic Acids

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