{"title":"INHIBITION BY LITHIUM OF GENTAMICININDUCED RELEASE OF N-ACETYL--DGLUCOSAMINIDASE IN ISOLATED PERFUSED RAT KIDNEY","authors":"M. Djamali, M. Shahrokhi","doi":"10.1080/107691897229766","DOIUrl":null,"url":null,"abstract":"N-Acetyl--d-glucosaminidase (NAG) is one of the sensitive hydrolytic lysosomal enzymes that is released after renal tubular damage. We have studied gentamicin-induced nephrotoxicity by determining the NAG release in a perfused rat kidney model. Gentamicin at 100 ug/ml caused a time-dependent increase in enzymuria. The effect of pretreatment with 5 or 10 mmol/kg of lithium chloride, administered subcutaneously 24 h prior to perfusion with gentamicin, was also studied through the measurement of NAG activity. Lithium at 5 mmol/kg absolutely inhibited NAG release; 10 mmol/kg lithium significantly diminished the release of enzyme. The inhibitory effect of 5 mmol/kg of lithium may be due to its interference with phosphoinositide cycle in renal tubular lysosomal membrane.","PeriodicalId":87425,"journal":{"name":"Toxic substance mechanisms","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxic substance mechanisms","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/107691897229766","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
N-Acetyl--d-glucosaminidase (NAG) is one of the sensitive hydrolytic lysosomal enzymes that is released after renal tubular damage. We have studied gentamicin-induced nephrotoxicity by determining the NAG release in a perfused rat kidney model. Gentamicin at 100 ug/ml caused a time-dependent increase in enzymuria. The effect of pretreatment with 5 or 10 mmol/kg of lithium chloride, administered subcutaneously 24 h prior to perfusion with gentamicin, was also studied through the measurement of NAG activity. Lithium at 5 mmol/kg absolutely inhibited NAG release; 10 mmol/kg lithium significantly diminished the release of enzyme. The inhibitory effect of 5 mmol/kg of lithium may be due to its interference with phosphoinositide cycle in renal tubular lysosomal membrane.