High-density linkage mapping and distribution of segregation distortion regions in the oak genome

C. Bodénès, É. Chancerel, F. Ehrenmann, A. Kremer, C. Plomion
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引用次数: 55

Abstract

We developed the densest single-nucleotide polymorphism (SNP)-based linkage genetic map to date for the genus Quercus. An 8k gene-based SNP array was used to genotype more than 1,000 full-sibs from two intraspecific and two interspecific full-sib families of Quercus petraea and Quercus robur. A high degree of collinearity was observed between the eight parental maps of the two species. A composite map was then established with 4,261 SNP markers spanning 742 cM over the 12 linkage groups (LGs) of the oak genome. Nine genomic regions from six LGs displayed highly significant distortions of segregation. Two main hypotheses concerning the mechanisms underlying segregation distortion are discussed: genetic load vs. reproductive barriers. Our findings suggest a predominance of pre-zygotic to post-zygotic barriers.
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橡树基因组分离扭曲区的高密度连锁定位与分布
我们开发了迄今为止栎属最密集的单核苷酸多态性(SNP)连锁遗传图谱。利用基于8k基因的SNP阵列对栎(Quercus pepeea)和栎(Quercus robur)两个种内和种间全同胞家族的1000多个全同胞进行了基因分型。在这两个物种的8个亲本图谱之间观察到高度共线性。然后,在橡树基因组的12个连锁群(LGs)上建立了4261个SNP标记,跨越742 cM的复合图谱。来自6个LGs的9个基因组区域显示出高度显著的分离扭曲。本文讨论了关于分离扭曲机制的两种主要假设:遗传负荷与生殖障碍。我们的研究结果表明,合子前到合子后的障碍占主导地位。
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