R. Shahjahan, Afroja Karim, R. Begum, M. S. Alam, A. Begum
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引用次数: 23
Abstract
The electrophoretic banding pattern of esterase isozymes were examined in eighteen different tissues of Nile tilapia after staining with I± and I² naphthyl acetate as substrate. The tissues were anterior-, mid-, tailmuscle (ventral and tip region), buccal muscle, stomach, fore-, mid-, hind-gut, liver, gill, heart, kidney, eye (lens), eye (iris), fore-, mid- and hind- brain. Altogether five bands named as Est-11.4, Est-21.1, Est-31, Est-40.62 and Est-50.25 were observed different relative mobility. Est-1 and Est-5 denote the fastest and slowest band. Est-3 was present in all the tissues. All five bands were expressed in liver and stomach. Some of the esterase bands showed tissue and substrate specificity, where Est-1 was in fore-, mid- and hind- brain, Est-2 in hind gut, Est-5 in stomach, gill and heart was stained only with I± naphthyl acetate. None of the band was expressed with I² naphthyl acetate only. Key words: esterase, isozyme, Oreochromis, tissue  Doi:10.3329/ujzru.v27i0.1942 Univ. j. zool. Rajshahi Univ. Vol. 27, 2008 pp. 01-05
以I±和I²乙酸萘酯为底物，对罗非鱼18种不同组织中酯酶同工酶的电泳带型进行了研究。组织为前、中、尾肌(腹侧和尖端)、颊肌、胃、前、中、后肠、肝脏、鳃、心脏、肾脏、眼(晶状体)、眼(虹膜)、前、中、后脑。Est-11.4、Est-21.1、Est-31、Est-40.62和Est-50.25 5个条带的相对迁移率不同。Est-1和Est-5表示最快和最慢的波段。所有组织中都有Est-3。5条条带均在肝脏和胃中表达。部分酯酶条带具有组织特异性和底物特异性，其中Est-1位于前、中、后脑，Est-2位于后肠，Est-5位于胃、鳃和心脏，仅用I±乙酸萘酯染色。没有一个条带仅用I²乙酸萘酯表达。关键词:酯酶，同工酶，Oreochromis，组织Â Doi:10.3329/ujzru.v27i0.1942Rajshahi university . Vol. 27, 2008 pp. 01-05
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