Enzymatic retting of jute ribbons

S. Majumdar, A.B. Kundu, S. Dey, B.L. Ghosh
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引用次数: 11

Abstract

Commercial enzyme preparations, viz. Pectinex ultra Sp-L and Flaxzyme consisting predominantly of pectinolytic enzymes were found to ret green jute ribbons within 48 h, producing fairly good quality jute fibres. The commercial enzyme Celluclast, consisting mainly of cellulase, hemicellulase and a relatively low proportion of pectinase was, on the other hand, ineffective in bringing about similar retting. A fiftyfold dilution of both Pectinex ultra Sp-L and Flaxzyme was able to ret ribbons of jute plants of 105–115 days old. producing fibres having a fineness in the range of 1.6–1.7 tex and a tenacity of bundles in the range of 23–25 g/tex. The fibres produced were totally free of barky root ends. Metal complexing agents such as citrate phosphate buffer and EDTA were found to stimulate enzymatic retting thereby helping to reduce effective enzyme concentration. The ret-liquor containing the retting end products were partially analysed. Amongst the monosaccharides, galacturonic acid was most predominant followed by glucose, arabinose, galactose and xylose; and amongst the gummy polysaccharides, pectin was most conspicuous.

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黄麻带的酶解
商业酶制剂,即Pectinex ultra Sp-L和主要由果胶分解酶组成的Flaxzyme,在48小时内就能使黄麻绿带分解,生产出质量相当好的黄麻纤维。另一方面,主要由纤维素酶、半纤维素酶和比例相对较低的果胶酶组成的商品酶Celluclast则不能产生类似的发酵效果。将Pectinex ultra Sp-L和Flaxzyme稀释50倍后,可以提取105-115天龄黄麻植株的条带。生产细度在1.6-1.7特克斯之间,成束强度在23 - 25g /特克斯之间的纤维。所生产的纤维完全没有树皮根尖。金属络合剂,如柠檬酸磷酸盐缓冲液和EDTA,被发现可以刺激酶还原,从而有助于降低有效酶浓度。对含发酵终产物的发酵液进行了部分分析。单糖中半乳糖醛酸含量最高,其次为葡萄糖、阿拉伯糖、半乳糖和木糖;胶性多糖中以果胶最为明显。
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