Haydeé Jaqueline Huayhua Mamani, R. G. García Castro
{"title":"Efecto antimicrobiano de la tunta (Solanum juzepczukii) sobre la Salmonella enterica subespecie enterica serovar Typhimurium","authors":"Haydeé Jaqueline Huayhua Mamani, R. G. García Castro","doi":"10.18271/RIA.2021.224","DOIUrl":null,"url":null,"abstract":"The objective of the study was to investigate the antimicrobial activity of tunta on Salmonella enterica subspecies enterica serovar Typhimurium. A natural isolate of this bacterium was used that was resistant to chloramphenicol. The experiment required the preparation of a standard solution of 0,8 g / ml of tunta extract. The Kirby Bauer technique was used for the antimicrobial evaluation of the tests and the Duraffourd scale to measure the level of sensitivity. Resulting, for the ten technical repetitions of the experimental group, an average halo of 10,40 mm and a standard deviation of 0,63 of DHI (level of limit sensitivity, according to said scale). In conclusion, our work shows evidence that tunta has an antimicrobial effect at a borderline sensitivity level on Salmonella enterica subspecies enterica serovar Typhimurium. After more in-depth research, this derivative of Solanum Jueepczukii could become an alternative for the development of antibiotics of natural origin and allow a sustainable development of Andean cultures.","PeriodicalId":41861,"journal":{"name":"Revista Investigaciones Altoandinas-Journal of High Andean Research","volume":"24 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2021-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Investigaciones Altoandinas-Journal of High Andean Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18271/RIA.2021.224","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The objective of the study was to investigate the antimicrobial activity of tunta on Salmonella enterica subspecies enterica serovar Typhimurium. A natural isolate of this bacterium was used that was resistant to chloramphenicol. The experiment required the preparation of a standard solution of 0,8 g / ml of tunta extract. The Kirby Bauer technique was used for the antimicrobial evaluation of the tests and the Duraffourd scale to measure the level of sensitivity. Resulting, for the ten technical repetitions of the experimental group, an average halo of 10,40 mm and a standard deviation of 0,63 of DHI (level of limit sensitivity, according to said scale). In conclusion, our work shows evidence that tunta has an antimicrobial effect at a borderline sensitivity level on Salmonella enterica subspecies enterica serovar Typhimurium. After more in-depth research, this derivative of Solanum Jueepczukii could become an alternative for the development of antibiotics of natural origin and allow a sustainable development of Andean cultures.
本研究旨在探讨金枪鱼对肠道沙门氏菌亚种肠炎血清型鼠伤寒杆菌的抑菌活性。这种细菌的天然分离物对氯霉素有抗性。该实验需要制备0.8 g / ml的金枪鱼提取物标准溶液。Kirby Bauer技术用于测试的抗菌评估,Duraffourd量表用于测量敏感性水平。结果,对于实验组的10次技术重复,平均光晕为10,40 mm, DHI(根据所述量表的极限灵敏度水平)的标准偏差为0.63。总之,我们的研究表明,金枪鱼对肠沙门氏菌亚种肠血清型鼠伤寒杆菌具有临界敏感性的抗菌作用。经过更深入的研究,这种Jueepczukii的衍生物可以成为开发天然抗生素的替代品,并允许安第斯文化的可持续发展。