Spaltung von löslicher ribonucleinsäure und serin-spezifischen transfer-ribonucleinsäure-fraktionen mit pankreas-ribonuclease

Abstract

Several RNA samples were split with pancreatic RNAase (polyribonucleotide 2-oligonucleotidotransferase (cyclizing), EC 2.7.7.16). The fragments were separated by chromatography on DEAE-cellulose columns and in some cases by subsequent paper electrophoresis. The amounts of mono- and dinucleotides from various unfractionated and partially fractionated soluble RNA's were compared (Table I). In hydrolysates of highly purified serine transfer RNA fractions 7 di-, 7 tri-, 7 tetra-, 2 penta- and 2 hexanucleotides - coming from 2 serine transfer-RNAs - were identified and quantitatively determined (Tables II and III), as well as some oligonucleotides, which occur in smaller amounts and probably come from contaminations of the serine transfer-RNA fractions. 3 oligonucleotides could not be identified, and one only partially. Several oligonucleotides contain odd nucleotides.