Seroprevalence and Biomarkers Detection of Human Herpes Virus (HHV-8) in Patients with Kaposi Sarcoma

Q4 Biochemistry, Genetics and Molecular Biology Journal of Biomolecular Techniques Pub Date : 2023-06-27 DOI:10.51173/jt.v5i2.857

Abdulrazzaq N. Zghair, S. Kasim, Nazar Sh. Mohammed, Anil K Sharma

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Abstract

Kaposi's sarcoma (KS) represents the most prevalent malignancy among untreated HIV-positive individuals. Herpesvirus linked with Kaposi's sarcoma (KSHV; also termed as human herpesvirus 8 (HHV8)). In this study, blood samples were collected from 120 individuals, 60 of them had HHV-8 infection with kaposi sarcoma and 60 persons as apparently healthy control. These patients attended Baghdad Teaching Hospital from the period of 15th February 2021 to 15th January 2022. Infections are seen to be more prevalent in the age group of 25-49 years when compared to other groups. The distribution of the biomarkers confirmed that 50, 25 and 80% of the 20 infected patients were positive for Ca19.9, Ca125 and Ca15.3 respectively. The Ca19.9, Ca125, and Ca15.3 biomarkers all produced good results in patients with Human Herpes Virus 8 (HHV-8) infections, leading us to the conclusion that these biomarkers gave favorable results. All of the PCR products showed a positive amplification at 434bp. Phylogenetic analysis confirmed the belongingness to the HHV strain. Further, this could lead to the development of a novel molecular diagnostic tool.

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人类疱疹病毒(HHV-8)在卡波西肉瘤患者中的血清阳性率和生物标志物检测

卡波西氏肉瘤(KS)是未经治疗的hiv阳性个体中最常见的恶性肿瘤。与卡波西肉瘤相关的疱疹病毒;也被称为人类疱疹病毒8 (HHV8))。本研究收集了120人的血液样本，其中60人感染了HHV-8卡波西肉瘤，60人作为表面健康对照。这些病人于2021年2月15日至2022年1月15日在巴格达教学医院就诊。与其他年龄组相比，25-49岁年龄组的感染更为普遍。生物标志物的分布证实，20例感染患者中Ca19.9、Ca125和Ca15.3分别为50%、25%和80%的阳性。Ca19.9、Ca125和Ca15.3生物标志物在人类疱疹病毒8 (HHV-8)感染患者中均产生良好的结果，这使我们得出结论，这些生物标志物具有良好的结果。所有PCR产物均在434bp处呈阳性扩增。系统发育分析证实该病毒属于HHV毒株。此外，这可能会导致一种新的分子诊断工具的发展。

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来源期刊

Journal of Biomolecular Techniques Biochemistry, Genetics and Molecular Biology-Molecular Biology

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2.50

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0.00%

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期刊介绍： The Journal of Biomolecular Techniques is a peer-reviewed publication issued five times a year by the Association of Biomolecular Resource Facilities. The Journal was established to promote the central role biotechnology plays in contemporary research activities, to disseminate information among biomolecular resource facilities, and to communicate the biotechnology research conducted by the Association’s Research Groups and members, as well as other investigators.