Screening of Lactobacillus from breast milk and infant feces and evaluation of their bile salt tolerance

Yujun Huang, Junjuan Yu, Haodong Yan, Chenchen Zhang, Wenli Kang, Lina Pan, Jiaqi Wang, Zhiyong Dai, R. Gu
{"title":"Screening of Lactobacillus from breast milk and infant feces and evaluation of their bile salt tolerance","authors":"Yujun Huang, Junjuan Yu, Haodong Yan, Chenchen Zhang, Wenli Kang, Lina Pan, Jiaqi Wang, Zhiyong Dai, R. Gu","doi":"10.15586/qas.v14i4.1053","DOIUrl":null,"url":null,"abstract":"The intestinal bile salt concentration of infants is lower than that of adults, and the necessary bile salt tolerance of probiotics for infants remains unclear. In this study, Lactobacillus strains were isolated from breast milk and infant feces. The strains with better bile salt tolerance were screened, and their bile salt tolerance was compared with Lactobacillus strains from the adult intestine. The results showed that the bile salt concentration had a better distinguishing effect when it was 0.075 and 0.1%. Among the 28 strains of Lactobacillus with better bile salt tolerance, there were 16 strains of Lactobacillus plantarum, among which eight strains had greater than 70% survival under 0.075% bile salt and two strains had more than 50% survival under 0.1% bile salt. L. plantarum strains iso-lated from breast milk and infant feces had a significantly lower survival rate than those isolated from the adult intestine (P < 0.05) under 0.1% bile salt, while there was no significant difference in the survival rate under 0.075% bile salt (P > 0.05). Therefore, the demands for bile salt tolerance of probiotics for infants might be lower than that for adults. It is suggested that the standard for the screening of probiotics from breast milk and infant feces should differ from adults.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Quality Assurance and Safety of Crops &amp; Foods","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15586/qas.v14i4.1053","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

The intestinal bile salt concentration of infants is lower than that of adults, and the necessary bile salt tolerance of probiotics for infants remains unclear. In this study, Lactobacillus strains were isolated from breast milk and infant feces. The strains with better bile salt tolerance were screened, and their bile salt tolerance was compared with Lactobacillus strains from the adult intestine. The results showed that the bile salt concentration had a better distinguishing effect when it was 0.075 and 0.1%. Among the 28 strains of Lactobacillus with better bile salt tolerance, there were 16 strains of Lactobacillus plantarum, among which eight strains had greater than 70% survival under 0.075% bile salt and two strains had more than 50% survival under 0.1% bile salt. L. plantarum strains iso-lated from breast milk and infant feces had a significantly lower survival rate than those isolated from the adult intestine (P < 0.05) under 0.1% bile salt, while there was no significant difference in the survival rate under 0.075% bile salt (P > 0.05). Therefore, the demands for bile salt tolerance of probiotics for infants might be lower than that for adults. It is suggested that the standard for the screening of probiotics from breast milk and infant feces should differ from adults.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
母乳和婴儿粪便中乳杆菌的筛选及其对胆盐耐受性的评价
婴儿肠道胆盐浓度低于成人,益生菌对婴儿胆盐的必要耐受性尚不清楚。本研究从母乳和婴儿粪便中分离出乳杆菌菌株。筛选了胆盐耐受性较好的菌株,并将其与成人肠道乳杆菌的胆盐耐受性进行比较。结果表明,胆盐浓度在0.075和0.1%时具有较好的鉴别效果。在28株胆盐耐受性较好的乳杆菌中,植物乳杆菌有16株,其中0.075%胆盐条件下8株存活率大于70%,0.1%胆盐条件下2株存活率大于50%。在0.1%胆盐条件下,母乳和婴儿粪便分离的植物乳杆菌的存活率显著低于从成人肠道分离的植物乳杆菌(P < 0.05),而在0.075%胆盐条件下,两者存活率无显著差异(P > 0.05)。因此,婴儿对益生菌胆盐耐受性的需求可能低于成人。建议从母乳和婴儿粪便中筛选益生菌的标准应与成人不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
A beetroot-based beverage produced by adding Lacticaseibacillus paracasei: an optimization study Safety evaluation of genetically modified crops Effect of pullulan active packaging, incorporated with silver nanoparticles, on cholesterol oxidation product concentrations in boiler meat during storage Sustainable rural economy and food security: An integrated approach to the circular agricultural model Mechanistic insight into ochratoxin A adsorption onto the cell wall of Lacticaseibacillus rhamnosus Bm01 and its impact on grape juice quality
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1