A. Garg, Nimisha Khurana, Ananya Chugh, Kangna Verma, Vandana Malhotra
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引用次数: 0
Abstract
Mycobacterium tuberculosis has the innate ability to adapt and survive the intracellular environments during infection. Two-component signalling (TCS) systems and serine (Ser)/threonine (Thr) protein kinases facilitate metabolic and growth adaptation by directing transcri-ptomic reprogramming in response to environmental stimuli. Presently, little is known about the post-transla-tional regulation of TCS proteins through O- phosphorylation. Using the NetPhosBac 1.0 in silico tool, we screened components of M. tuberculosis TCS systems for potential Ser/Thr phosphosites. We report extensive Ser/Thr phosphorylation of sensor kinases and response regulator proteins, suggesting that it might be a distinct mechanism enabling the co-regulation of pathways impac-ting adaptive changes in mycobacterial growth and meta-bolism.
结核分枝杆菌在感染期间具有适应和存活细胞内环境的先天能力。双组分信号(TCS)系统和丝氨酸(Ser)/苏氨酸(Thr)蛋白激酶通过指导转录-情景重编程来响应环境刺激，促进代谢和生长适应。目前，对TCS蛋白通过O-磷酸化的翻译后调控知之甚少。使用NetPhosBac 1.0 in silico工具，我们筛选结核分枝杆菌TCS系统的组分，以寻找潜在的Ser/Thr磷酸位点。我们报道了传感器激酶和反应调节蛋白广泛的丝氨酸/苏氨酸磷酸化，这表明它可能是一种独特的机制，能够共同调节影响分枝杆菌生长和代谢适应性变化的途径。
期刊介绍：
Current Science, published every fortnight by the Association, in collaboration with the Indian Academy of Sciences, is the leading interdisciplinary science journal from India. It was started in 1932 by the then stalwarts of Indian science such as CV Raman, Birbal Sahni, Meghnad Saha, Martin Foster and S.S. Bhatnagar. In 2011, the journal completed one hundred volumes. The journal is intended as a medium for communication and discussion of important issues that concern science and scientific activities. Besides full length research articles and shorter research communications, the journal publishes review articles, scientific correspondence and commentaries, news and views, comments on recently published research papers, opinions on scientific activity, articles on universities, Indian laboratories and institutions, interviews with scientists, personal information, book reviews, etc. It is also a forum to discuss issues and problems faced by science and scientists and an effective medium of interaction among scientists in the country and abroad. Current Science is read by a large community of scientists and the circulation has been continuously going up.
Current Science publishes special sections on diverse and topical themes of interest and this has served as a platform for the scientific fraternity to get their work acknowledged and highlighted. Some of the special sections that have been well received in the recent past include remote sensing, waves and symmetry, seismology in India, nanomaterials, AIDS, Alzheimer''s disease, molecular biology of ageing, cancer, cardiovascular diseases, Indian monsoon, water, transport, and mountain weather forecasting in India, to name a few. Contributions to these special issues ‘which receive widespread attention’ are from leading scientists in India and abroad.
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