PRELIMINARY VALIDATION OF UV SPECTROPHOTOMETRIC FOR DETERMINATION OF ANTIEMETIC DRUG APREPITANT IN BULK FORM

Abstract

Objective: The main objective of this work was to put forth the assorted strategies to develop and validate a novel, specific, precise and reliable method for estimation of aprepitant in bulk using UV-visible spectroscopy method. Method: The validation of Aprepitant was done by using UV-visible spectrophotometric method by using double beam systronics UV-visible spectrometer, model UV-2201 (India). The validation method involves various parameters like linearity, precision, accuracy, robustness, ruggedness, detection, quantification limits of formulation analysis according to International Conference on Harmonization (ICH) guidelines. Results: UV-spectroscopic determination was carried out at maximum absorption 263.6nm using pH 6.8 buffer & 1.1% tween 80 and 263.8nm using methanol and distilled water. The method obeyed Beer Lambert’s Law in the concentration range of 8-48µg/ml and R2 was found to be 0.999. Conclusion: As per the results were concerned, the %RSD was found to be less than 2% which is compliance with the acceptance criteria of Q1 (R1) and According to results, the currently developed method shows compliance with acceptance criteria with Q1 (R1) and international conference on harmonization (2005) guidelines. Thus, the developed method was found to be simple accurate and précised.