Amino acid balance of microbial-tissue complex in the small intestinal and liver under administration of lead acetate

Abstract

It is known that the initiating event of liver damage during lead intoxication is the effect of lead on the intestinal microbiome and the metabolic profile of enterocytes. The aim of the study was to reveal the dependence of the concentrations of free amino acids and their derivatives in the liver on the amino acid pool of the microbial-tissue complex of the small intestine. We used rats weighing 120‒140 g, which were injected intragastrically for three weeks with drinking water with lead acetate. Free amino acids and their nitrogen-containing metabolites in the microbial-tissue complex of the intestine and liver were determined by HPLC. Enteral intake of lead acetate with drinking water changes the profile of free amino acids and their derivatives in the microbial-tissue complex of the small intestine, which correlates with a violation of amino acid homeostasis in the liver. The analysis of the correlations of the indicators we determined indicates the essential role of nitrogen-containing metabolites of amino acids ‒ ethanolamine and phosphoethanolamine, taurine, and cystathionine. The direction of the correlation relationship between various nitrogen-containing metabolites of the microbial-tissue complex and the liver can be considered as marker of discoordination of homeostasis.