Anti-Cryptococcus neoformans antibodies during cryptococcosis in patients with the acquired immunodeficiency syndrome

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1995-12-01 DOI:10.1016/0888-0786(96)87297-0

F. Dromer , D.W. Denning , D.A. StevenS , A. Noble , J.R. Hamilton

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引用次数: 7

Abstract

We studied the anti-cryptococcal IgG antibody response in patients with the acquired immunodeficiency syndrome (AIDS) after Cryptococcus neoformans infection. The reactivity of 64 sera from 16 AIDS patients with cryptococcosis and 70 from noninfected HIV-negative individuals was measured against purified capsular polysaccharide by enzyme-linked immunosorbent assay (ELISA) or whole cryptococci by flux cytometry (FACS). AIDS patients had higher titres of antibodies by ELISA than controls. FACS analysis using the infecting isolate suggested antibody production was lower when the infecting isolate was serotype D. Lack of correlation between ELISA and FACS suggest antibodies produced during infection have a different specificity than that of normals. We did not find a correlation between antibody levels and clinical course. The lack of correlation between antibody and antigen titres even after pretreatment with pronase showed the proteinaceous substance unmasked by pronase is not composed only of specific antibodies.

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获得性免疫缺陷综合征患者隐球菌感染期间的抗新型隐球菌抗体

我们研究了获得性免疫缺陷综合征(AIDS)患者感染新型隐球菌后抗隐球菌IgG抗体的反应。采用酶联免疫吸附试验(ELISA)和流式细胞术(FACS)分别检测了16例艾滋病隐球菌感染者和70例非hiv感染者的64份血清对纯化荚膜多糖的反应性。ELISA检测的艾滋病患者抗体滴度高于对照组。使用感染分离物进行的FACS分析表明，当感染分离物为血清型d时，抗体产生较低，ELISA和FACS之间缺乏相关性，表明感染期间产生的抗体与正常人具有不同的特异性。我们没有发现抗体水平与临床病程之间的相关性。即使在pronase预处理后，抗体和抗原滴度之间也缺乏相关性，这表明被pronase揭开的蛋白质物质并不仅仅由特异性抗体组成。

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Serodiagnosis and Immunotherapy in Infectious Disease

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