{"title":"DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance","authors":"Peng Zheng, Ziming Wang, Xiaoyu Li, Fushuo Huang, Mingjun Ma, He Huang","doi":"10.3906/vet-1910-100","DOIUrl":null,"url":null,"abstract":"The purpose of this experiment was to investigate the effects of Beltsville thawing solution BTS , Androhep, and Dulbecco?s modified Eagle medium + Fatal bovine serum D-F as diluents of frozen semen on sperm motility and sow reproductive performance. In experiment 1, boar semen was collected and diluted to 2.0 × 109/60 mL, 1.5 × 109/60 mL, and 1.5 × 109/40 mL with Androhep. The appropriate semen dose was determined by postcervical insemination. In experiment 2, boar semen was fumigated with liquid nitrogen and thawed semen was diluted to 1.5 × 109/40 mL with D-F, Androhep, and BTS, respectively. The effects of above diluents on sperm motility and sow reproductive performance were studied by postcervical insemination. The appropriate semen dose for postcervical insemination was 1.5 × 109/40 mL. After thawing of frozen semen, sperm motility of semen diluted with D-F was significantly higher than that of semen diluted with Androhep and BTS. There was no significant difference in pregnancy rates among sows via artificial insemination. The litter size of sows using D-F diluted semen was significantly higher than that of sows using Androhep and BTS diluted semen. In conclusion, D-F freezing diluent can improve sperm motility of frozen boar semen after thawing, prolong sperm survival time, and increase sow reproductive performance.","PeriodicalId":23357,"journal":{"name":"Turkish Journal of Veterinary & Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish Journal of Veterinary & Animal Sciences","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3906/vet-1910-100","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The purpose of this experiment was to investigate the effects of Beltsville thawing solution BTS , Androhep, and Dulbecco?s modified Eagle medium + Fatal bovine serum D-F as diluents of frozen semen on sperm motility and sow reproductive performance. In experiment 1, boar semen was collected and diluted to 2.0 × 109/60 mL, 1.5 × 109/60 mL, and 1.5 × 109/40 mL with Androhep. The appropriate semen dose was determined by postcervical insemination. In experiment 2, boar semen was fumigated with liquid nitrogen and thawed semen was diluted to 1.5 × 109/40 mL with D-F, Androhep, and BTS, respectively. The effects of above diluents on sperm motility and sow reproductive performance were studied by postcervical insemination. The appropriate semen dose for postcervical insemination was 1.5 × 109/40 mL. After thawing of frozen semen, sperm motility of semen diluted with D-F was significantly higher than that of semen diluted with Androhep and BTS. There was no significant difference in pregnancy rates among sows via artificial insemination. The litter size of sows using D-F diluted semen was significantly higher than that of sows using Androhep and BTS diluted semen. In conclusion, D-F freezing diluent can improve sperm motility of frozen boar semen after thawing, prolong sperm survival time, and increase sow reproductive performance.
期刊介绍：
The Turkish Journal of Veterinary and Animal Sciences is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK).
Accepts English-language manuscripts on all aspects of veterinary medicine and animal sciences.
Contribution is open to researchers of all nationalities.
Original research articles, review articles, short communications, case reports, and letters to the editor are welcome.
Manuscripts related to economically important large and small farm animals, poultry, equine species, aquatic species, and bees, as well as companion animals such as dogs, cats, and cage birds, are particularly welcome.
Contributions related to laboratory animals are only accepted for publication with the understanding that the subject is crucial for veterinary medicine and animal science.
Manuscripts written on the subjects of basic sciences and clinical sciences related to veterinary medicine, nutrition, and nutritional diseases, as well as the breeding and husbandry of the above-mentioned animals and the hygiene and technology of food of animal origin, have priority for publication in the journal.
A manuscript suggesting that animals have been subjected to adverse, stressful, or harsh conditions or treatment will not be processed for publication unless it has been approved by an institutional animal care committee or the equivalent thereof.
The editor and the peer reviewers reserve the right to reject papers on ethical grounds when, in their opinion, the severity of experimental procedures to which animals are subjected is not justified by the scientific value or originality of the information being sought by the author(s).
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