Irradiated peripheral blood mononuclear cells for assessing individual radiosensitivity in laryngeal cancer radiotherapy

A. Amaral, Heberton Ferreira, Thaisa Feliciano de Souza, J. Melo, Amanda Iumatti Santos Firmo Xavier, Edvane Borges da Silva, Luciano Rodolfo Ferreira de Lucena, André Maciel Netto
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Abstract

In laryngeal cancer radiotherapy (LCRT), dermatitis, dysphagia, and odynophagia are common acute side effects, varying in intensity depending mainly on the patient's radiosensitivity. This study investigated the survival rate of in vitro irradiated peripheral blood mononuclear cells (PBMC) as a pre-therapeutic test to assess individual radiosensitivity and predict the degree of these acute side effects in LCRT. 16 patients diagnosed with early-stage laryngeal cancer undergoing radiotherapy without adjuvant chemotherapy participated as volunteers in this study. Before the beginning of treatment, a sample of PBMC was collected and irradiated with 2.5 Gy using a 6 MV linear accelerator. After 72-h cell incubation, the trypan blue exclusion test was performed to score viable cells through optical microscopy. The percentages of cell viability were compared to the intensity of acute side effects experienced by LCRT patients. Even though no correlation was found regarding the observed degrees of radiation dermatitis, our results indicate that patients with survival rates of in vitro irradiated PBMC higher than 70% are more likely to experience moderate to severe acute odynophagia and dysphagia after LCRT. The proposed and discussed methodology points to a practical and affordable pre-therapeutic test to evaluate individual radiosensitivity in order to prevent or reduce acute side effects in laryngeal cancer radiotherapy.
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辐照外周血单个核细胞评估喉癌放疗中个体放射敏感性
在喉癌放疗(LCRT)中,皮炎、吞咽困难和吞咽困难是常见的急性副作用,其强度主要取决于患者的放射敏感性。本研究考察了体外辐照的外周血单个核细胞(PBMC)的存活率,作为评估LCRT患者个体放射敏感性和预测这些急性副作用程度的治疗前试验。16例早期喉癌患者接受放疗,不辅助化疗,作为志愿者参与本研究。在治疗开始前,收集PBMC样品,使用6 MV直线加速器以2.5 Gy照射。细胞孵育72 h后,进行台盼蓝排斥试验,通过光学显微镜对活细胞进行评分。将细胞存活率的百分比与LCRT患者所经历的急性副作用的强度进行比较。尽管观察到的放射性皮炎程度没有相关性,但我们的研究结果表明,体外辐照PBMC存活率高于70%的患者在LCRT后更容易出现中度至重度急性吞咽困难和吞咽困难。提出和讨论的方法指向一种实用和负担得起的治疗前试验,以评估个体放射敏感性,以预防或减少喉癌放疗的急性副作用。
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